Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4092587..4093163 Replicon chromosome
Accession NZ_CP045277
Organism Escherichia coli strain LAU-OXA

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag GBF15_RS19920 Protein ID WP_001297643.1
Coordinates 4092876..4093163 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag GBF15_RS19915 Protein ID WP_000063156.1
Coordinates 4092587..4092889 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GBF15_RS19895 4088609..4088806 - 198 Protein_3843 Tar ligand binding domain-containing protein -
GBF15_RS19900 4089183..4091333 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
GBF15_RS19905 4091383..4091586 + 204 WP_000467859.1 YbdD/YjiX family protein -
GBF15_RS19910 4091597..4092553 + 957 WP_032296927.1 GTPase -
GBF15_RS19915 4092587..4092889 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
GBF15_RS19920 4092876..4093163 - 288 WP_001297643.1 BrnT family toxin Toxin
GBF15_RS19925 4093362..4094059 - 698 Protein_3849 IS1 family transposase -
GBF15_RS19930 4094116..4094211 + 96 Protein_3850 dienelactone hydrolase family protein -
GBF15_RS19935 4094357..4095589 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
GBF15_RS19940 4095630..4096910 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - cheD 4087389..4093865 6476


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T141629 WP_001297643.1 NZ_CP045277:c4093163-4092876 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T141629 NZ_CP060049:1937135-1937237 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT141629 WP_000063156.1 NZ_CP045277:c4092889-4092587 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT141629 NZ_CP060049:c1937244-1937099 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References