Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2464490..2465128 Replicon chromosome
Accession NZ_CP045277
Organism Escherichia coli strain LAU-OXA

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag GBF15_RS11965 Protein ID WP_000813794.1
Coordinates 2464952..2465128 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag GBF15_RS11960 Protein ID WP_001270286.1
Coordinates 2464490..2464906 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GBF15_RS11940 2459642..2460583 - 942 WP_001251304.1 ABC transporter permease -
GBF15_RS11945 2460584..2461597 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
GBF15_RS11950 2461615..2462760 - 1146 WP_131199864.1 ABC transporter substrate-binding protein -
GBF15_RS11955 2463005..2464411 - 1407 WP_069905165.1 PLP-dependent aminotransferase family protein -
GBF15_RS11960 2464490..2464906 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
GBF15_RS11965 2464952..2465128 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
GBF15_RS11970 2465350..2465580 + 231 WP_000494244.1 YncJ family protein -
GBF15_RS11975 2465672..2467633 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
GBF15_RS11980 2467706..2468242 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
GBF15_RS11985 2468295..2469509 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T141625 WP_000813794.1 NZ_CP045277:c2465128-2464952 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T141625 NZ_CP060049:326132-326500 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGTCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT141625 WP_001270286.1 NZ_CP045277:c2464906-2464490 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT141625 NZ_CP060049:325914-326135 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References