Detailed information of TA system TA141623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2317870..2318396
• Replicon: chromosome
• Accession: NZ_CP045277
• Organism: Escherichia coli strain LAU-OXA

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: GBF15_RS11235
• Protein ID: WP_000323025.1
• Coordinates: 2318109..2318396 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: GBF15_RS11230
• Protein ID: WP_000534858.1
• Coordinates: 2317870..2318109 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBF15_RS25495	2312879..2313166	+	288	Protein_2158	hypothetical protein	-
GBF15_RS11210	2313935..2315248	+	1314	WP_069905218.1	ISNCY family transposase	-
GBF15_RS11215	2315698..2316906	-	1209	WP_000343717.1	IS256 family transposase	-
GBF15_RS11220	2317005..2317337	-	333	WP_069905221.1	FlxA-like family protein	-
GBF15_RS11225	2317540..2317845	-	306	WP_001326990.1	hypothetical protein	-
GBF15_RS11230	2317870..2318109	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
GBF15_RS11235	2318109..2318396	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
GBF15_RS11240	2318468..2318623	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
GBF15_RS11245	2318840..2319091	+	252	WP_000980994.1	hypothetical protein	-
GBF15_RS11250	2319158..2319436	+	279	WP_012304870.1	hypothetical protein	-
GBF15_RS11255	2319438..2320487	+	1050	WP_001265198.1	DUF968 domain-containing protein	-
GBF15_RS11260	2320501..2321253	+	753	WP_001047135.1	antitermination protein	-
GBF15_RS11265	2321531..2321620	-	90	WP_120795389.1	hypothetical protein	-
GBF15_RS11270	2321675..2321887	-	213	WP_000087756.1	cold shock-like protein CspF	-
GBF15_RS11275	2322188..2322403	+	216	WP_000066484.1	cold shock-like protein CspB	-
GBF15_RS11280	2323157..2323372	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2308053..2334461	26408
	-	flank	IS/Tn	-	-	2315698..2316906	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T141623 WP_000323025.1 NZ_CP045277:2318109-2318396 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T141623 NZ_CP060047:c338275-337988 [Vibrio vulnificus]
ATGAAAGTAGTTTGGTCTCCATTAGCGCTTCAGAAGCTAGGCGATGCAGCGGAGTTTATTTCTTTGGATAATCCATCTGC
GGCAGAAAAGTGGGTAAATGAAGTTTTTGATAAAACCGAACTACTTGGAAGCATGCCTGAAATGGGGCGCTTTGTTCCTG
AAATGCCCCATACGAACTATCGTGAAATCATCTTTGGTCACTATCGGATTATCTACAGCTTAAGTCACGAAATCCGCGTG
CTAACAGTTCGTAATTGTCGCCAAATACTGTCTGAAGATGACGTGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT141623 WP_000534858.1 NZ_CP045277:2317870-2318109 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT141623 NZ_CP060047:c338553-338272 [Vibrio vulnificus]
ATGAGTCGTATTCATTTCGATCAAGATATTCAGCCGTTATCTGAATTTCGTGCAGGTGTTGCTTCTTACATCAAACAAAT
CAATGAGACTCGCCGTCCATTGGTCATTACACAGCGTGGTAAAGGTGTTGCTGTTGTTCTTGATGTTGCAGAGTATGAAG
CAATGCAAGAGAAAATCGAATTACTAGAAGAAATGCGCACAGCTGAAGCTCAATTAGCTTCAGGCCTTGGTGTTTCCAAT
GATGATGCTCGTGCTCAAGTACTGGGGCGCATTAAGAAATGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8