Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 892271..892925 Replicon chromosome
Accession NZ_CP045277
Organism Escherichia coli strain LAU-OXA

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag GBF15_RS04360 Protein ID WP_000244777.1
Coordinates 892518..892925 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GBF15_RS04355 Protein ID WP_000354046.1
Coordinates 892271..892537 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GBF15_RS04330 887440..888183 + 744 WP_000951964.1 SDR family oxidoreductase -
GBF15_RS04335 888240..889673 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
GBF15_RS04340 889718..890029 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
GBF15_RS04345 890193..890852 + 660 WP_000250274.1 hemolysin III family protein -
GBF15_RS04350 891048..892028 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
GBF15_RS04355 892271..892537 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GBF15_RS04360 892518..892925 + 408 WP_000244777.1 toxin CptA Toxin
GBF15_RS04365 892965..893486 - 522 WP_001055874.1 flavodoxin FldB -
GBF15_RS04370 893598..894494 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GBF15_RS04375 894519..895229 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GBF15_RS04380 895235..896968 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T141612 WP_000244777.1 NZ_CP045277:892518-892925 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T141612 NZ_CP060042:c47035-46625 [Burkholderia cenocepacia]
ATGACGCTGTATTTGCTCGACACCAACATACTGTCGAACGTGATCCGCGACCCGCGCGGCGCATGCGCCACGCGGATTGG
AGAGACCCCGCCGGAGCAAGTGTGCACCTCGATCGTCGTCGCTGCGGAACTGCGATTCGGCGTCTGGAAGCGCGGCTCGT
CAACGCTCGCGCAGCGCGTCGAGCAACTGCTCGCGAGCTTGACCGTGTTGCCGCTGCAGCCCGACGCGGACCGCTGCTAC
GGGCGGCTGCGGGCCGAGCTCGAAAAGCAAGGGCAGCTGATCGGCGCCAACGACATGCTGATCGCCGCTCACGCGCTGGC
CGTCGACGCCGTGCTGGTCACCGACAACACGGCTGAATTCACCCGAGTCGCCGGGCTCCCGGTCGAAAACTGGCTGCGAT
CGGCAACGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT141612 WP_000354046.1 NZ_CP045277:892271-892537 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT141612 NZ_CP060042:c47274-47032 [Burkholderia cenocepacia]
ATGCCAACCCATGACGCCCGCCACGCGCGCTTGTTTCGCAACGGGCGCAGCCAGGCCGTGCGGATCCCGCGCGAATTCGA
GCTGCCGGTCTCGGAGGTCACCATCCACCGGGAAGGCCGCCGCTTGATCATTGAACCCGTGGAAGCGGCACCCTTGCGTG
AGCTGTTCGCCCAATGGGCCCCGCTGGACGAAACCTTTCCGGACATCGAGGACCTTCCGCCTGAGCCGGTGGACATCGAA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References