Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 73336..73979 Replicon plasmid p16HN-263_KPC
Accession NZ_CP045264
Organism Klebsiella pneumoniae strain 16HN-263

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag ELO34_RS28770 Protein ID WP_001044770.1
Coordinates 73336..73752 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag ELO34_RS28775 Protein ID WP_001261282.1
Coordinates 73749..73979 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ELO34_RS28745 69439..69711 - 273 Protein_92 transposase -
ELO34_RS28750 69740..70437 - 698 Protein_93 IS1 family transposase -
ELO34_RS28760 70693..71715 - 1023 WP_000361404.1 helicase UvrD -
ELO34_RS28765 71700..73262 - 1563 WP_004206609.1 AAA family ATPase -
ELO34_RS28770 73336..73752 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
ELO34_RS28775 73749..73979 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ELO34_RS28780 73936..74397 + 462 WP_014343465.1 hypothetical protein -
ELO34_RS28790 74558..75502 + 945 WP_011977810.1 hypothetical protein -
ELO34_RS28795 75539..75931 + 393 WP_011977811.1 hypothetical protein -
ELO34_RS28800 75989..76510 + 522 WP_013214008.1 hypothetical protein -
ELO34_RS28805 76556..76759 + 204 WP_011977813.1 hypothetical protein -
ELO34_RS28810 76789..77793 + 1005 WP_011977814.1 hypothetical protein -
ELO34_RS28820 77977..78756 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2 / catA2 - 1..129252 129252


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T141604 WP_001044770.1 NZ_CP045264:c73752-73336 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T141604 NZ_CP060040:c1254481-1253570 [Burkholderia cenocepacia]
ATGAGTGTCATCGACAAACGTGAACGCGACCGCACCGCGGCGTTCGCCGCGCTGCTCGGGCAGTTTCCCGAGCAGCGTTG
CGCGGCCGGCGCGGCGGGCACGATTGGCTACCGTGAGGCGGGTGCAGGCGAGGCCGGCGCGCAGCATGCGGGCCGTGCGC
TGCCCGTCGTGCTGCTGCACGGGATCGGTTCGGGCGCCGCATCGTGGGTCCGCCAGCTCGACGCGCTCGGTGCATCGCGG
CGTGTACTCGCGTGGGATGCGCCCGGTTACGGCGTGTCGACGCCCGTGCACGGCGCGTCGCCCGCCGCCGCCGATTACGC
GGCATCGCTGAACGCGTGGCTCGAGGCACTCGGCATCGAACGCTGCGTGCTGGTCGGTCATTCGCTCGGCGCGATCATCG
CGGGCGGCCTCGTCCGCGTGATGCCCGCGCGGATCGCCGGGCTGCTGCTGATTTCGCCCGCGGGCGGCTACGGCAGTGCG
CCAGCCGACACGCGCGAATCGCGCCGCGACGCACGGCTCGCGATGCTCGCGGAACTCGGCCCGGCCGGGCTCGCCGAGCA
GCGCAGCGGCAACATGCTGTCCGGCTTCGCGAACGAGGAGGCAAAGGCATGGGTGCGCTGGAACATGGCGCGCATCGTGC
CGGCCGGCTATGCGCAGGCCACGCATCTGCTCGCGAACGCCGATCTCGCGGCCGATCTCGCCGGCTTTCGCGGCCGCACG
GCCGTGGCCGTCGGCGCGAACGACGCGATCACGCCGCCCGCCGCGTGCGAGCGGATCGCCGCGGCCGCGCACGTCGGGCT
GCAGGTGATTCCGCAGGCAGGGCATGCCGGCTACGTGGAAGCGCCGGCCGTTTATTCCGCGCTGATCGATACGTTCTGTC
GTCAGTGCGACCCACAGCGGGGGCTGGCATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT141604 WP_001261282.1 NZ_CP045264:c73979-73749 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT141604 NZ_CP060040:c1253573-1252716 [Burkholderia cenocepacia]
ATGAGCGAACCGCTGCAACAAAACGAATCCGACCACGCGAAGGCGGAAGCCAGCTACGTCGTGCCGGGCCTCGAACGCGG
GCTGCGGATTCTCGCCGAATTCTCGCCGCGCGAGCCCGTGCTCGGCGCGCCCGAGCTGTCGAAACGGCTCGGCATTCCGC
GCACGACGGTGTTCCGTCTGCTGCAGACGCTCGAATCGCTCGGTTTTCTCGAACGCGCGGACAAGGATCGCAACTACAAG
CTCGGCATCGCCGTGCTGCGGCTCGGCTTCGAATACCTGAGCTCGCTGGAACTGACCGATCTCGGCCTGCCGGTGATCGA
GAGCCTGCGCGACGCGACCGGCTTCACGACGCACATCGTGATTCGCGACGGCCGCGACGTGGTGTTCGTCGCGAAGGCGC
AGAGCCAGTCGCCGGTGTTCAGCTCGATCCGCGTGAACGTCGGCACCCGGCTGCCCGCGCACGCGACGACGCACGGCCAC
GTGCTGATGGGCGACCTGTCGCTGAAGGAACTGCACGCGCTGTATCCGGAAGGCGCGCTGAACCGGATGACGAGCGCGAC
GCCGGAAACGGTCGACGCGCTGTACGACGTGATCCGCGAGGATGCGCTGCGCGGCTACGGCGTCAGCAATTCGTCGTTCG
AGCGCGGGATCTCGGTCGTCACGGCGCCGGTACGCAACGAGACGCAGAGGATCGTCGCGTGCATCACGGTGACGGTGCCG
CGTCCGGAGATCGACGCGGCGCTGATCGCGGACGGGCTGATCGACAAGGTGCAGCGCGCAGCGGCGGAATTGTCGCGGCG
GCTCAATTACCGCTCGGATGACGAGCACACGTTTCTCAAAGCTTTAGGACTCCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References