Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5520982..5521607 Replicon chromosome
Accession NZ_CP045263
Organism Klebsiella pneumoniae strain 16HN-263

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag ELO34_RS27875 Protein ID WP_002882817.1
Coordinates 5520982..5521365 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ELO34_RS27880 Protein ID WP_004150355.1
Coordinates 5521365..5521607 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ELO34_RS27860 5518348..5519250 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
ELO34_RS27865 5519247..5519882 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
ELO34_RS27870 5519879..5520808 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ELO34_RS27875 5520982..5521365 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
ELO34_RS27880 5521365..5521607 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
ELO34_RS27885 5521812..5522729 + 918 WP_002882812.1 alpha/beta hydrolase -
ELO34_RS27890 5522743..5523684 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
ELO34_RS27895 5523729..5524166 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
ELO34_RS27900 5524163..5525023 - 861 WP_002882807.1 virulence factor BrkB family protein -
ELO34_RS27905 5525017..5525616 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T141599 WP_002882817.1 NZ_CP045263:c5521365-5520982 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T141599 NZ_CP060040:854684-855097 [Burkholderia cenocepacia]
ATGAGCAGGATTGAGACGGAGAAATCATTCGTCGACAGCAATGTCGTGCTGTATCTGCTGTCCGAGGACGAAACCAAGGC
AAACCGTGCGGAGTCGCTGTTCCTGCGGCGGCCGACCATCAGCGTCCAGGTACTGAACGAAGTCGCCAGTGTGTGCAACC
GCAAATTGCGGATGCCGTGGCGCGAGGTAGGGAAGTTTTTGGAGCCGATCAGGAGTTTGTGCCATGTCGTACCGCTCACC
GTCGATGCGCACGACCTCGCGCGGCGGCTGGCAGAGTGGCATCGCTTGTCCTTCTACGATGCATGCATCGTGGCAACTGC
CTCAATCGAAGGATGTGAATACCTGTACACCGAAGACATGCACGACGGCTTGCAAGTGGACGGCGGTCCGGTATTGCGAA
ATCCGTTTGCCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT141599 WP_004150355.1 NZ_CP045263:c5521607-5521365 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT141599 NZ_CP060040:854464-854697 [Burkholderia cenocepacia]
ATGCAGGTCGCAAAATGGGGCAATAGCCTGGCGGTTCGCCTTCCGGCCAGTGTGGTTGACGCACTGGAACTCCGCGAAGG
CGACGATATTGAGATAGTGGTCGACAGCCCGCGGGTTTTCGCGGTAAGTCGCAAGCCGAGGCCCGACGAGCTTCTGGAGC
GACTGCGGCGTTTCAGGGGCAAATTGCCCGCCGATTTCAAGTTCAGCCGGGACGATGCGAATGAGCAGGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References