Detailed information of TA system TA141597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 5145957..5146627
• Replicon: chromosome
• Accession: NZ_CP045263
• Organism: Klebsiella pneumoniae strain 16HN-263

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: ELO34_RS26075
• Protein ID: WP_004213072.1
• Coordinates: 5145957..5146400 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: ELO34_RS26080
• Protein ID: WP_004213073.1
• Coordinates: 5146397..5146627 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ELO34_RS26040	5141369..5141644	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
ELO34_RS26045	5141707..5142198	+	492	WP_011251327.1	DM13 domain-containing protein	-
ELO34_RS26050	5142247..5143167	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
ELO34_RS26055	5143258..5143664	+	407	Protein_4998	GAF domain-containing protein	-
ELO34_RS26060	5144179..5144813	-	635	Protein_4999	response regulator	-
ELO34_RS26065	5145230..5145523	+	294	Protein_5000	transposase	-
ELO34_RS26070	5145533..5145943	+	411	WP_196976738.1	restriction endonuclease	-
ELO34_RS26075	5145957..5146400	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
ELO34_RS26080	5146397..5146627	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ELO34_RS26085	5147235..5148368	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
ELO34_RS26090	5148384..5148677	+	294	WP_004213076.1	hypothetical protein	-
ELO34_RS26095	5148667..5148873	-	207	WP_004213077.1	hypothetical protein	-
ELO34_RS26100	5149225..5149515	+	291	WP_004213078.1	hypothetical protein	-
ELO34_RS26105	5149505..5150404	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	mph(E)	iucA / iucB / iucC / iucD / iutA / rmpA	5051895..5201973	150078

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T141597 WP_004213072.1 NZ_CP045263:c5146400-5145957 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T141597 NZ_CP060040:c154641-154342 [Burkholderia cenocepacia]
ATGGACAAGATCAAGACGCGGCCGTGGGATTCGGCCGAACACCTGAAAACCGAAGACGACATGGCCGACTACTTCGACGC
CTGCCTGCAGGAGGCCGGCGACGATCCGGCCTTCATCGCGCATGCGCTCGGCGTGATCGCCCGTGCCCGCGGCATGTCGC
AGGTCGCACGCGATGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAATTGCACGGCGCCAGGGCTGCCGCATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT141597 WP_004213073.1 NZ_CP045263:c5146627-5146397 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT141597 NZ_CP060040:c154983-154645 [Burkholderia cenocepacia]
ATGGTTGACACCCTGCAATCCGTGCCCTACAGTGCGCCTACGTTCAGCATCCGGACCACCGACGTCTTCGAAGCGTGGTT
CGCCGGCCTGCCGGATCGCGTCGCGCGGCGGCGCATCCAGGCGCGCATCGACCGTCTGTCGATGGGCAATCCGGGCGACT
GGAAATCCGCCGGCTCCCCCGTCGTCGAAATGCGTATCGACCACGGCCCCGGCTATCGCGTCTATTACGTGAGGCGCGGA
ACGATATGGGTCATCCTGCTCTGCGGCGGCGATAAGTCGACGCAACAGGCCGACATCCGCGCCGCGCACGCAATGCTCGC
GCACCTCGACATGGAGTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620