Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4828598..4829408 Replicon chromosome
Accession NZ_CP045263
Organism Klebsiella pneumoniae strain 16HN-263

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag ELO34_RS24440 Protein ID WP_002887280.1
Coordinates 4828598..4829131 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag ELO34_RS24445 Protein ID WP_002887278.1
Coordinates 4829142..4829408 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ELO34_RS24435 4827429..4828550 + 1122 WP_002887282.1 cupin domain-containing protein -
ELO34_RS24440 4828598..4829131 - 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
ELO34_RS24445 4829142..4829408 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
ELO34_RS24450 4829511..4830944 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
ELO34_RS24455 4830934..4831617 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
ELO34_RS24460 4831789..4833174 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
ELO34_RS24465 4833192..4833536 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T141594 WP_002887280.1 NZ_CP045263:c4829131-4828598 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T141594 NZ_CP060037:84204-85478 [Sphingobium barthaii]
GTGAGCACCGACATTCTCGATCGTCTTATCGTCTGGTCCGACCAGCATATCGCGGGCGAACTCTCCATCGACCGTGCAGG
CGCGATGCGTTTCAGCTACGCGCCGGACTGGCTCGCCGATCCCGATAGAACAGCGCTCTCGCAGGCCATGCCCAAGAGGG
TTGAGTCATTTGGCGATGCGCTGTGCAAGGCCGTCTTCGGCGGGCTGCTGCCCGAGGAAGGGCAACGGACCGCCATCGCC
CGCGCCCTCGGTGTATCGCCCGACAATCCCTTTCGCCTGTTGGCGGCGCTTGGCGGCGACGTTGCCGGCGCCCTGGCCTT
TTTGCCGGAAGGGGAAATGCCGCCAGCGGCACCCGCCGGCCGGCCGGCTCGCCCGCTTGATGACACAGAACTGGCGGCGC
TGATCGCGCGTTTGCCGCGCGTACCGATGCTGGCCGGGGAGGGGGGCGCACGGCTCAGTCTGGCCGGCGCGCAAAGCAAG
TTGCCCGTCGTTCTGGTCAAGGGCGCCATCGCCTTGCCGCGTGTCGGCGAGCCATCGACCCATCTCATCAAGTCCGAGCC
CGATCGCTTTCCGGGACTGGCGGCCAATGAGGCATTCTGTCTCGCCCTGGCGCGATCGGTTGGGCTCGATGCGGTCATGG
CCGAGTGGCGCGCCGTCGCTGGGAAGGGCTATCTCCTGGTGACCCGCTACGATCGCACCGTACAGCCCGGCGCGACCTCG
CGCCTGCATCAGGAGGACTTCGCACAGGCGCTGGGCGTACCGTCCAACCGGAAATATGCGAGCGAGGGCGGGCCGACGTT
CCGAGACGGCTTCGCGCTCGTCCGCGCGGCCACTTCACGGCCGGCCCGCGAGGTGCTGAAACTCGCCGATGCCGCGATCT
TCAATGTGATCATCGGCAATGCCGACGCGCATGCCAAAAACTACAGTCTGCTTCGCCGCGCCAACGGCGAGGTGGTCCTT
GCCCCCCTCTACGATCTGGTTGCGACCCATGTCTGGCCGGAGCTGTCGCCAAAGCTCGCCATGCGATTTGGGCGTGCCGC
GACGCTCGAGGATCTGGACGCTGCAAGCTTTGTACGCTTTGCGCAGGATGCCGGTCTCGCGCTTCCCTTCCTGCGCCGGC
GGGCCGCGGCGATCGCGGCGCGCGTGCAGGCTGCGATTACCGATCGCGTCGAGATCCCCGGGCTGGAGGATACCGCTTGT
CTGGAGGACCTGCCGGCAACCGTGCTCGACCGGGCGATGCGGTTGTCACTGAAAGCGGAAGCGAGAGGCCGCTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT141594 WP_002887278.1 NZ_CP045263:c4829408-4829142 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT141594 NZ_CP060037:83974-84207 [Sphingobium barthaii]
ATGCTCGACATGATAACCATCGGCCGGATCGTTCGCGACGAACGCAAGGAACTGGGACTGCGTCAGGATGAACTGGCGGC
AGCAAGCGGCGTGGGACTGCGCTTTATCGTCGAGGTCGAGCGGGGTAAGCCGACGGCGCAGATCGGCAAGGTCATCGCGG
TCCTGGCCGCGCTCGGCTGCGAGCTTGAGATCCGCCGGCCGGACGGCATCGTGATCGCCGGCCGGCCTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References