Detailed information of TA system TA141578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 52142..52676
• Replicon: plasmid pCD
• Accession: NZ_CP045260
• Organism: Yersinia pestis strain SCPM-O-B-5942 (I-2638)

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q663N8
• Locus tag: GD372_RS22570
• Protein ID: WP_002213360.1
• Coordinates: 52377..52676 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q9ZGW3
• Locus tag: GD372_RS22565
• Protein ID: WP_002224342.1
• Coordinates: 52142..52384 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GD372_RS22545	48842..50008	+	1167	WP_002220902.1	plasmid-partitioning protein SopA	-
GD372_RS22550	50005..50970	+	966	WP_002213354.1	ParB/RepB/Spo0J family plasmid partition protein	-
GD372_RS23235	51425..51598	-	174	WP_002213357.1	hypothetical protein	-
GD372_RS22560	51684..51976	+	293	Protein_64	IS6 family transposase	-
GD372_RS22565	52142..52384	+	243	WP_002224342.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
GD372_RS22570	52377..52676	+	300	WP_002213360.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GD372_RS22575	52816..53475	-	660	WP_002229754.1	type III secretion system effector GTPase activator YopE	-
GD372_RS22580	53669..54061	+	393	WP_002213403.1	type III secretion system chaperone SycE/YerA	-
GD372_RS22585	54124..54768	-	645	Protein_69	IS3 family transposase	-
GD372_RS22590	54871..56043	+	1173	WP_002220893.1	IS21 family transposase	-
GD372_RS22595	56043..56441	+	399	Protein_71	ATP-binding protein	-
GD372_RS22600	56818..57243	+	426	WP_002213267.1	type III secretion system chaperone SycH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	yopO/ypkA / yopJ/yopP / yopH / lcrQ/yscM / yscL / yscK / ylpB/yscJ / lcrO/yscI / yopR/yscH/lcrP / yscG / yscF / yscE / yscD / yscC / yscB / virG/yscW / yscU / yscT / yscS / yscR / yscQ / yscP / yscO / yscN / lcrE/yopN / tyeA / sycN / yscX / yscY / yscV/lcrD / lcrR / lcrG / lcrV / sycD/lcrH / yopB / yopD / yopM / sycT / yopT / yopK/yopQ / yopE / sycE/yerA / sycH / yadA / yadA	1..68252	68252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (5-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11632.19 Da
• Isoelectric Point: 5.5114

>T141578 WP_002213360.1 NZ_CP045260:52377-52676 [Yersinia pestis]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNPELGAECNWICSDIRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDVQDFPEHE

Nucleotide

• Length: 300 bp

>T141578 NZ_CP060016:1108179-1108628 [Mycolicibacterium boenickei]
ATGGCTAAATTGTCTGTCTCCGTCGATGTTCCATTGCCGCCGGAGAAGGCGTGGGAGTACGCCTCTGATCTGTCCCGGTA
CGACGAGTGGCTCAGCATCCACCGCGCCTGGCGGTCGAAGTTGCCCGAGACCCTGGAAAAGGGCACGGTCATCGACTCGA
TCGTCGAAGTCAAGGGCATGCTGAACCGTGTGAAGTGGACGCTGGTGAATTACAAGCCACCGCAGTCCCTGACCCTCAAC
GGCGATGGCCGTGGCGGGGTAAAGGTCAAGCTGATCGGAAAGATCGCTCCGGCCCAGGTTGACGGCGGCGAGGGCGCGAA
AGTGTCCTTCGACGTGCATCTAGGAGGGCCTGCCCTGTTCGGGCCGATCGGCATGGTGGTCGCGGCCGCGCTCAAGGGCG
ATATCCAGCAGTCGCTGAACAAGTTCAAGGCGCTCTACGCGTCGTCGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8865.15 Da
• Isoelectric Point: 6.4781

>AT141578 WP_002224342.1 NZ_CP045260:52142-52384 [Yersinia pestis]
MAHVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNVPVSHRLMDEIFSAAVKDTSV

Nucleotide

• Length: 243 bp

>AT141578 NZ_CP060016:1107996-1108175 [Mycolicibacterium boenickei]
ATGGGATTCCTGGACAAGGCCAAGGACCTTCTGTCTCAGAACGCGGACAAGGTAGAGCAGGCCATCGACAAGGCCGGCGA
CATCGTCGACGAGAAGACGCAGGGCAAGTACTCCGGCGCGGTCGACAAGGCACAGGAAGCGGCGAAGAACGCCATCAACA
AGGAAGAACCGCAGCAGTAA

Structures

Toxin

• AlphaFold DB: Q663N8

Antitoxin

• AlphaFold DB: A0A0E1NQY6