Detailed information of TA system TA141558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3197419..3198440
• Replicon: chromosome
• Accession: NZ_CP045257
• Organism: Proteus mirabilis strain L90-1

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: GCE56_RS15630
• Protein ID: WP_004249488.1
• Coordinates: 3197871..3198440 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: GCE56_RS15625
• Protein ID: WP_004245528.1
• Coordinates: 3197419..3197871 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCE56_RS15605	3193205..3193735	-	531	WP_004245524.1	RNA pyrophosphohydrolase	-
GCE56_RS15610	3194004..3195017	+	1014	WP_004250228.1	thiamine ABC transporter substrate binding subunit	-
GCE56_RS15615	3195017..3196597	+	1581	WP_004245526.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
GCE56_RS15620	3196590..3197291	+	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
GCE56_RS15625	3197419..3197871	+	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
GCE56_RS15630	3197871..3198440	+	570	WP_004249488.1	PIN domain-containing protein	Toxin
GCE56_RS15635	3198556..3200910	+	2355	WP_017628571.1	DNA polymerase II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21600.10 Da
• Isoelectric Point: 7.3470

>T141558 WP_004249488.1 NZ_CP045257:3197871-3198440 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKNPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T141558 NZ_CP060015:c605690-605433 [Mycobacterium malmoense]
GTGAGGCTGGTCTTCACGCCTCTCGGATGGGCCGACTACCAGTACTGGAGCACAACCGACCGAGGGACACAGAAGCGTAT
CAACCGCCTCATCGATGACACCTTGCGTGATCCGTTCGCCGGGATTGGCAAACTTGAGCCGTTACGCCACGCCTTCGCGG
GCGCCTGGTCACGGCGCATCAGCGAGGAACACCGCTTGGTGTACCTCGTCGATGGCGATGACGTCGTGATCGTGCAGGCC
CGGTACCACTACACCTGA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT141558 WP_004245528.1 NZ_CP045257:3197419-3197871 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT141558 NZ_CP060015:c605932-605687 [Mycobacterium malmoense]
GTGGCGATCACCGCGTCCGAGGCACGCAAGAACTTATTTCCGTTGATCGAGCAGGTCAACAACGACCGCGTCCCGGTGGA
GATCAACTCCCGCCGGGGTGATGCGGTATTGATCTCGCGCGCCGACTACGAAGCCCTGGAAGAGACCGCGCACCTGTTGC
GGTCCCCCGCCAATGCCCGTCGGCTGCTGGAAAGCCTTACCCAGGCGCAGCGTGGCGAACGCGAAGAGCACACGTTGTCC
CGGTGA

Structures

Antitoxin

• AlphaFold DB: B4F2H3