Detailed information of TA system TA141548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1087471..1088048
• Replicon: chromosome
• Accession: NZ_CP045245
• Organism: Cellulomonas sp. JZ18

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GC089_RS05085
• Protein ID: WP_155376728.1
• Coordinates: 1087698..1088048 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GC089_RS05080
• Protein ID: WP_155376727.1
• Coordinates: 1087471..1087701 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GC089_RS05060	1083007..1083656	+	650	Protein_996	response regulator transcription factor	-
GC089_RS05065	1083820..1084164	+	345	WP_155376724.1	hypothetical protein	-
GC089_RS05070	1084238..1084732	+	495	WP_155376725.1	DUF456 domain-containing protein	-
GC089_RS05075	1084856..1087414	+	2559	WP_155376726.1	DNA helicase PcrA	-
GC089_RS05080	1087471..1087701	+	231	WP_155376727.1	ribbon-helix-helix domain-containing protein	Antitoxin
GC089_RS05085	1087698..1088048	+	351	WP_155376728.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GC089_RS05090	1088230..1089411	+	1182	WP_155378942.1	ADP-forming succinate--CoA ligase subunit beta	-
GC089_RS05095	1089450..1090337	+	888	WP_155376729.1	succinate--CoA ligase subunit alpha	-
GC089_RS05100	1090606..1091892	+	1287	WP_196250828.1	hypothetical protein	-
GC089_RS05105	1091915..1092556	-	642	WP_155376730.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12501.35 Da
• Isoelectric Point: 8.7706

>T141548 WP_155376728.1 NZ_CP045245:1087698-1088048 [Cellulomonas sp. JZ18]
VSRRGDVWLVDLDPARGREASRTRPVVVVSNQAAVTSAFAADRGVVTVVPLTSNVARVLPFQAYLPASDTGLEVDSKAQA
EQVRSVDVGRFVELVGVVPPVLMRDVDRALRLHLSL

Nucleotide

• Length: 351 bp

>T141548 NZ_CP060009:1674866-1675618 [Pseudomonas sediminis]
ATGGACATCTGGCAACTATGCGACGGCGAGCGACAGGTGCGACCGCTGCGCGGCAAGCTGTTGCGCATGGTGGAAAGCCA
GGCGCAGGTCGCGACCCTGCAACTGGTGGATACCCTCGCCGAGCAGGCCCTGCTCGAGGAGTTGCTCGAGAACAGCAAAC
CGCCGCTGCCGGAGGCTACCGAGCCGCTGCACTATCTGTTGAAGACGCCGTTCCGTTATCCGCCGCTGCGCTGGGGTTCG
CGCTTCGGCACCGTACACGAACCCAGCCTGTTCTACGCCGCCCAGCGCCTTGAAACGGCGATGGCCGAGGCTGCCTACTA
CCGTTTCGTACTGTGGAATGGCATGGACACTGCGCCGCCCAGTGGCCGCATCCTCTCCGAGCATTCCACCTTCGAGGCGC
GTTATCGCGTCGAGCGCGGTATCCAGTTGCATAAGCCACCCTTCGAGGGGCATGGCGTCCTGTTGGCTCACCCCAGCGAC
TACAGCGTGCCCCAGCGCCTCGGTGCGGCGATGCGTGCAGCGGGCATCCAGGCCTTCGAGTACCGCTCGGCGCGCTGCCC
GGATGGTGGCAGCAATGTCGCGCTCTATGTGCCGGCTGCGTTTGCGGAGAAGCGCCCGCGCAATCTGACCGCCTGGTTGT
GTGAAACCACGGGTGAGTACGTGGCGTTCAAGCATGCGCAAACGCCGGATCAACCGCGGCTGTTTCACTGGGAGCAGTTT
CTGGTGCAAGGGCAACTGCCTCACCCCGCGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8329.05 Da
• Isoelectric Point: 4.0713

>AT141548 WP_155376727.1 NZ_CP045245:1087471-1087701 [Cellulomonas sp. JZ18]
MKLSVSLPEGDVATLDAYVERHGTTRSGALHEAVRLLRERNLVEQYDEAFREWDGSDDAVAWDGAVADGLDQGAPE

Nucleotide

• Length: 231 bp

>AT141548 NZ_CP060009:1674418-1674789 [Pseudomonas sediminis]
ATGAGTGCAGTGACTCGCAGCCAGCCTGACGCCGATGCGGTACTGGCCAAGGCCTTGCTCAATACTCGTGAGCACCTGGG
GCTGACCCAGCAGGAGTTGGCCGATATCGTCGGCGTCCATCGTAGTGCCATCAGCCGCTGGAACGAGCAGGGCGGTTTGC
GCCCGCAAAGCAAGACCGGCGAACTGGCCCTGCTGTTGATTCGCGCCTACCGCGCGTTGTTCGCCCTGTTTGGCGGCAAT
CAGCAGGACATGCGCCATTTCCTGCGCACCGAGAATCGCCATCTGGCTGGCGTACCGCTGCAGCAGATGAGTCAGGTGCA
GGGGTTGGTGCGTGTCGTCGAGTATCTGGACGCCATTCGCGGCAAGGTCTGA