Detailed information of TA system TA141545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1804194..1804835
• Replicon: chromosome
• Accession: NZ_CP045236
• Organism: Burkholderia cepacia strain BC16

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BURCE16_RS25050
• Protein ID: WP_042973286.1
• Coordinates: 1804194..1804532 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U2GAF3
• Locus tag: BURCE16_RS25055
• Protein ID: WP_021157290.1
• Coordinates: 1804536..1804835 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BURCE16_RS25040	1800751..1802184	-	1434	WP_153489866.1	aldehyde dehydrogenase family protein	-
BURCE16_RS25045	1802214..1803854	-	1641	WP_027790992.1	acetolactate synthase large subunit	-
BURCE16_RS25050	1804194..1804532	+	339	WP_042973286.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BURCE16_RS25055	1804536..1804835	+	300	WP_021157290.1	putative addiction module antidote protein	Antitoxin
BURCE16_RS25060	1804892..1806178	-	1287	WP_060065763.1	DUF445 domain-containing protein	-
BURCE16_RS25065	1806390..1807610	-	1221	WP_153489867.1	FAD-dependent oxidoreductase	-
BURCE16_RS25070	1807600..1807926	-	327	WP_006479527.1	non-heme iron oxygenase ferredoxin subunit	-
BURCE16_RS25075	1807947..1808432	-	486	WP_059684523.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
BURCE16_RS25080	1808450..1809721	-	1272	WP_059694307.1	Rieske 2Fe-2S domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (26-98)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12637.52 Da
• Isoelectric Point: 9.5686

>T141545 WP_042973286.1 NZ_CP045236:1804194-1804532 [Burkholderia cepacia]
MVDIPQSMPYSAPTFSIRTTDVFDAWFAGLRDRVAKRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRG
TIWVILLCGGDKSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 339 bp

>T141545 NZ_CP060008:3775898-3776191 [Pseudomonas sp. B11D7D]
ATGGCATACCTCATCCAGCAGACAAAGACCTTCGCCAGCTGGCACTCCGCCGTACGCGATCTCAAGGCCAAGGTTGCAAT
CGCGCGCCGTATTGATCGAGCCGCCGCGGGCAACCTGGGTGATGTGAAGTCTGTAGGTGATGGAGTGTCTGAAATGCGCA
TTGATGTAGGCGCTGGCTACCGCCTGTACTTCACCGTCCGTGGCGGCATAGTCATTGTTCTGTTGGTAGGCGGCGACAAG
AGCACACAACCTGCTGATATCCGGCAGGCCAAGAAACTGGCAAAGGAGGTTTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10633.01 Da
• Isoelectric Point: 6.3217

>AT141545 WP_021157290.1 NZ_CP045236:1804536-1804835 [Burkholderia cepacia]
MDKISTRPWDSADHLKTEDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Nucleotide

• Length: 300 bp

>AT141545 NZ_CP060008:3776194-3776484 [Pseudomonas sp. B11D7D]
ATGCCTCATACCCTGATCCCATTCGACATGGCTGAGCTGCTCGACAGTGAAGAGGCAGTCAGCGAGTACCTGTCTCAAGT
CCTGGCCGACGGCGACACTGATGAACTGATCCGCGCGATCGGCCATGTGGCCAAAGCACGCGGCATGTCGCAGATAGCCA
AGGACTCTGGCCTGGGTCGTGCGAGTCTCTACAAGGCCCTCGCCCCCGGCGCGCAACCACGGTTCGATACCGTGATCAAG
GTCATGCATGCCCTGGGCATTGAACTGCACGCTACCCCGGCCCATACCTGA

Structures

Antitoxin

• AlphaFold DB: U2GAF3