Detailed information of TA system TA141543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1432168..1432745
• Replicon: chromosome
• Accession: NZ_CP045236
• Organism: Burkholderia cepacia strain BC16

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BURCE16_RS23405
• Protein ID: WP_059683095.1
• Coordinates: 1432380..1432745 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8B2Q139
• Locus tag: BURCE16_RS23400
• Protein ID: WP_048026026.1
• Coordinates: 1432168..1432386 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BURCE16_RS23375	1427395..1427877	+	483	WP_153489725.1	GNAT family N-acetyltransferase	-
BURCE16_RS23380	1427965..1429230	-	1266	WP_153489726.1	Zn-dependent hydrolase	-
BURCE16_RS23385	1429223..1429747	-	525	WP_153489727.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
BURCE16_RS23390	1429802..1430968	-	1167	WP_153489728.1	branched-chain amino acid ABC transporter substrate-binding protein	-
BURCE16_RS23395	1431146..1432042	+	897	WP_021160899.1	LysR family transcriptional regulator	-
BURCE16_RS23400	1432168..1432386	+	219	WP_048026026.1	hypothetical protein	Antitoxin
BURCE16_RS23405	1432380..1432745	+	366	WP_059683095.1	type II toxin-antitoxin system VapC family toxin	Toxin
BURCE16_RS23410	1433112..1433675	+	564	WP_153489729.1	peroxiredoxin	-
BURCE16_RS23415	1433804..1435402	+	1599	WP_059552487.1	alkyl hydroperoxide reductase subunit F	-
BURCE16_RS23420	1435498..1436292	-	795	WP_153489730.1	extensin	-
BURCE16_RS23425	1436453..1437006	-	554	Protein_1248	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13567.64 Da
• Isoelectric Point: 8.4826

>T141543 WP_059683095.1 NZ_CP045236:1432380-1432745 [Burkholderia cepacia]
MVKALFDTNILIDYLGGVESARVELARYSYRAISIISWMEVLVGTSAHDEATTRAWLSSFDVIALDSTIANRAVTIRQER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPATEPGIRMPYRI

Nucleotide

• Length: 366 bp

>T141543 NZ_CP060008:2425723-2426475 [Pseudomonas sp. B11D7D]
ATGGACATCTGGCAACTATGTGAGGGCGAGCGACAGGTGCGACCGCTGCGCGGCAGGCTGCTGCGCATGGTGGAAAGTCA
GGCGCAGGTCGCGACCCTGCAACTGGTGGATAACCTCGCCGAGCAAGCGCTGCTCGAAGAGTTGCTCGAGAGCAGCAAGC
CGCCGCTGCCCGAAGCCGCCGAGCCGTTGCACTATCTGTTGAAGACGCCATTTCGTTATCCACCACTGCGCTGGGGCTCG
CGCTTTGGCAGCGTGCATGAGCCCAGCCTGTTCTACGCCGCCCAGCGTCTGGAAACGGCGATGGCCGAGGCCGCCTACTA
TCGCTTCGTGCTGTGGAACGGCATGGCCACGCCGCCGCCCAGCGGGCGCATCCTCTCCGAACATTCCACTTTCGAGGCGC
GCTATCGCGTCGAGCGCGGTATTCAGTTGCAGCAGCCGCCGTTCGATCAGCATGGCACCCTGTTGGCGCACCCCAGTGAC
TACAGCGTGCCTCAGCGCCTCGGCACGGCAATGCGTGCGGCCGGTATCCAGGCCTTCGAGTATCGCTCGGCGCGCTGCCC
GGACGGCGGTAGCAATGTCGCGCTCTACGTGCCGGCCGCCTTCGCGGAAAAACGGCCGCGTAACCTGACGGCCTGGCTGT
GCGAAACCACGGGCGACTACGTGGCGTTCAAGCATGCGCAGACGCCGGATCAGCCTCGGCTGTTTCACTGGGAGCAGTTC
CTGGTGCAGGGGCAGCTGCCGCACCCCGCATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8257.35 Da
• Isoelectric Point: 4.4419

>AT141543 WP_048026026.1 NZ_CP045236:1432168-1432386 [Burkholderia cepacia]
MSRILVDLSDGQLDELAAIVETERRPRAAIIRDAIDAYIALHKRPLADDAFGLWKDRTVDGLAYQEEMRSEW

Nucleotide

• Length: 219 bp

>AT141543 NZ_CP060008:2425275-2425646 [Pseudomonas sp. B11D7D]
ATGAGTGCAGTGACTCGCAGCCAGCCTGACGCCGATGCGGTGCTGGCCAAGGCCTTGCTTAATACCCGTGAACAGTTGGG
GCTGACCCAGCAGGAGCTGGCCGATATCGTCGGCGTGCATCGCAGCGCCATCAGCCGCTGGAACGAGCAGGGCGGTTTGC
GCCCGCAGAGCAAGACGGGTGAACTGGCCCTGTTGTTGATTCGCGCCTACCGCGCTCTGTTCGCCCTGTTCGGCGGCAAC
CAGCAGGACATGCGCCACTTTCTGCGTACCGAGAACCGCCACCTGGCCGGCGTGCCGCTGCAGCAGATGGGGCAGGTGCA
GGGCTTGGTACGCGTCGTCGAGTATCTGGACGCCATTCGTGGCAAGGTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A8B2Q139