Detailed information of TA system TA141542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1223656..1224695
• Replicon: chromosome
• Accession: NZ_CP045236
• Organism: Burkholderia cepacia strain BC16

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: BURCE16_RS22550
• Protein ID: WP_153489643.1
• Coordinates: 1223656..1224231 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A806V2D4
• Locus tag: BURCE16_RS22555
• Protein ID: WP_027791436.1
• Coordinates: 1224228..1224695 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BURCE16_RS22525	1218973..1220040	+	1068	WP_021164062.1	ABC transporter ATP-binding protein	-
BURCE16_RS22530	1220200..1221291	+	1092	WP_136469117.1	porin	-
BURCE16_RS22535	1221462..1222427	-	966	WP_153489642.1	zinc-binding alcohol dehydrogenase family protein	-
BURCE16_RS22540	1222474..1222725	-	252	WP_006479165.1	hypothetical protein	-
BURCE16_RS22545	1222821..1223600	+	780	WP_027791438.1	helix-turn-helix transcriptional regulator	-
BURCE16_RS22550	1223656..1224231	-	576	WP_153489643.1	PIN domain-containing protein	Toxin
BURCE16_RS22555	1224228..1224695	-	468	WP_027791436.1	helix-turn-helix domain-containing protein	Antitoxin
BURCE16_RS22560	1224805..1225611	-	807	WP_059683325.1	4-hydroxy-2-oxoheptanedioate aldolase	-
BURCE16_RS22565	1225624..1226427	-	804	WP_111941667.1	2-oxo-hepta-3-ene-1,7-dioic acid hydratase	-
BURCE16_RS22570	1226448..1226840	-	393	WP_027791434.1	5-carboxymethyl-2-hydroxymuconate Delta-isomerase	-
BURCE16_RS22575	1226874..1227722	-	849	WP_027791433.1	3,4-dihydroxyphenylacetate 2,3-dioxygenase	-
BURCE16_RS22580	1227772..1229235	-	1464	WP_153489644.1	5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21421.72 Da
• Isoelectric Point: 5.2686

>T141542 WP_153489643.1 NZ_CP045236:c1224231-1223656 [Burkholderia cepacia]
MTSSPFTAVYDACVLYPAPLRDFLMWLALSGCFRARWSVDIHDEWKRNLLCNRADLTAAQLDRTASLMDRAIPDALVTGY
EALIGGLTLPDANDRHVLAAAIRCNASVIVTFNQKDFPEEVLSIYGVEAQHPDVFVDNLFDLEPAAVVAAARRQRETLKH
PPMIAERYLEILQRLGMVQTCKALEAYRTII

Nucleotide

• Length: 576 bp

>T141542 NZ_CP060003:60564-60890 [Xanthomonas citri pv. citri]
ATGATGCGGGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAAAACCAAGGCCCGCTCTGGTGATCCAGGCCGA
CCAGTTTGACGCACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTCGTTGCTGCGCCGCTGCTGCGTATTACCG
TTCATCCAAGTACCGACAATGGTTTGCAGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
GTAGGTCGAGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17497.87 Da
• Isoelectric Point: 6.2956

>AT141542 WP_027791436.1 NZ_CP045236:c1224695-1224228 [Burkholderia cepacia]
MPHTTMSETILPVEREMRAAVEGQRALAAYLATRTEIQRIQIFDDENRAHQVELPTSALRLLVDILAELADGNAVKVVPV
HAELTTQEAADLLNVSRPHLIKLLESNALPHHKTGKHRRVRFSDLMRYKAERDQASADAMEELSRQAQEHGMGYE

Nucleotide

• Length: 468 bp

>AT141542 NZ_CP060003:60397-60567 [Xanthomonas citri pv. citri]
ATGGCAGGGCTGCGTCCGGTGCAAATTTGGGTGCCGGACACACGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTG
TCGTCTTGCTGCACAAGCGGACATGGCGGATACCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCT
GGACGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A806V2D4