Detailed information of TA system TA141536

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 161950..162587
• Replicon: chromosome
• Accession: NZ_CP045235
• Organism: Burkholderia cepacia strain BC16

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BURCE16_RS00740
• Protein ID: WP_059687888.1
• Coordinates: 162186..162587 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A4S5D069
• Locus tag: BURCE16_RS00735
• Protein ID: WP_059528333.1
• Coordinates: 161950..162186 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BURCE16_RS00725	157544..158575	+	1032	WP_059687891.1	helix-turn-helix domain-containing protein	-
BURCE16_RS37945	158741..159838	-	1098	WP_194290280.1	ADP-heptose--LPS heptosyltransferase	-
BURCE16_RS37950	159839..161536	-	1698	Protein_139	thiamine pyrophosphate-binding protein	-
BURCE16_RS00735	161950..162186	+	237	WP_059528333.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BURCE16_RS00740	162186..162587	+	402	WP_059687888.1	type II toxin-antitoxin system VapC family toxin	Toxin
BURCE16_RS00745	162674..164062	-	1389	WP_021164187.1	L-serine ammonia-lyase	-
BURCE16_RS00750	164093..165229	-	1137	WP_059490381.1	alginate lyase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-120)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14812.86 Da
• Isoelectric Point: 4.8997

>T141536 WP_059687888.1 NZ_CP045235:162186-162587 [Burkholderia cepacia]
MARYMLDTNMCIYLMKNQPEQVARRFAQCYTGDVVMSAITYAELEYGVTACASPARERSNLAALIEDIPVAPFDAPAAQA
YGPVREAARDRKKDHLDKLIAAHAVSLDVVLVTNNERDFASYPGVRLENWLSD

Nucleotide

• Length: 402 bp

>T141536 NZ_CP060002:c2619199-2618786 [Xanthomonas citri pv. citri]
ATGGCCGCGGGTAAGGGCAAAGTGTTTCTCGACAGCAACGTGGTGCTCTACCTGCTGTCGGAGGATGCGGTGAAGGCGGA
CGGCGCGGAGGCATTGCTGCAGCGCAGGCCCGTCATCAGTGTGCAGGTGCTCAATGAAGTGACCCACGTCTGCGTTCGCA
AGCTCAAGATGGGCTGGGACGAAGTCGGGCAGTTCCTTGCGTTGGTGCGATCGTTCTGCAAGATAGTGCCTCTGACGGTC
GATGTTCACGACCGTGCGCGCCAGCTGGCGGAGCGGCACCAATTGTCGTTCTACGACGCGTGCATCGTGGCGGCGGCCGC
CATCGAAGGGTGCCAGACCCTCTACTCCGAAGACATGCACCACGGCCTCATCATCGAAGAGAGCCTGTCGATTCGGAACC
CGTTCAACGTCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8983.13 Da
• Isoelectric Point: 5.7252

>AT141536 WP_059528333.1 NZ_CP045235:161950-162186 [Burkholderia cepacia]
MHTTRVFRNGNSQAVRIPADLAYERSDIELEIERIGDEIRIRPMRRPLTGVLEKFAKFGPDFMAEGRGDHEQADREGL

Nucleotide

• Length: 237 bp

>AT141536 NZ_CP060002:c2619419-2619186 [Xanthomonas citri pv. citri]
ATGCAAGTCGCGAAGTGGGGTAACAGCCTGGCGGTGCGCCTGCCGTCCAGTCTGGTGGAAGCGCTGGAACTGCGCGAGGG
CGACGACATCGAGATCGTCGTCGATGATCCGCGATTGTTCGCGGTGCGCAAGAAGCCGGGTCCGGAGGCCATGTTGGAGC
GGCTGCGTGCCTTCCGGGGCAAGTTGCCCGCAGATTTCAAGTTCAACCGGGACGAGGCCAATGGCCGCGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A4S5D069