Detailed information of TA system TA141521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5191636..5192225
• Replicon: chromosome
• Accession: NZ_CP045221
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain ARS 38

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: FD951_RS23425
• Protein ID: WP_023969316.1
• Coordinates: 5191926..5192225 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: FD951_RS23420
• Protein ID: WP_007924096.1
• Coordinates: 5191636..5191929 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FD951_RS23395	5187176..5188339	-	1164	WP_023969313.1	acyltransferase	-
FD951_RS23400	5188534..5189724	-	1191	WP_023969314.1	serine hydrolase	-
FD951_RS23405	5189851..5190738	+	888	WP_009051074.1	LysR family transcriptional regulator	-
FD951_RS23415	5191110..5191502	+	393	WP_023969315.1	hypothetical protein	-
FD951_RS23420	5191636..5191929	-	294	WP_007924096.1	putative addiction module antidote protein	Antitoxin
FD951_RS23425	5191926..5192225	-	300	WP_023969316.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FD951_RS23430	5192397..5192846	+	450	WP_023969317.1	DUF3828 domain-containing protein	-
FD951_RS23435	5192871..5193881	-	1011	WP_193725262.1	NAD(P)-dependent alcohol dehydrogenase	-
FD951_RS23440	5193937..5194236	-	300	WP_009051080.1	ArsR family transcriptional regulator	-
FD951_RS23445	5194298..5195389	-	1092	WP_023969319.1	nitrite reductase, copper-containing	-
FD951_RS23450	5195615..5195857	+	243	WP_007924102.1	exodeoxyribonuclease VII small subunit	-
FD951_RS23455	5195854..5196741	+	888	WP_023969320.1	(2E,6E)-farnesyl diphosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (6-91)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11104.77 Da
• Isoelectric Point: 10.3417

>T141521 WP_023969316.1 NZ_CP045221:c5192225-5191926 [Pseudomonas chlororaphis subsp. aurantiaca]
MIEIKQTATFMEWENRLKDSRARALIAARIFRLANGLAGDVSPVGEGVSELRIHYGPGYRVYVQQLGSQLVILLCGGDKS
RQSRDIELAKQLARSWSRS

Nucleotide

• Length: 300 bp

>T141521 NZ_CP059997:c3767773-3767402 [Amycolatopsis sp. CA-230715]
ATGGCCTGGGAGATCCAGCTGCACCCCGAGGTGACCGCCTGGTTCCTGGAGTTGGTTCAAGAGGATCCGGACAGCGCGGA
TCTGGTGGAACACGCAATCGATCTCCTCGCGGAGGAAGGGCCTTCGCTCGGAAGGCCGCTGGCGGACCGCATCAAGGGCT
CGCGCATCCACAACATGAAGGAACTTCGGCCAGCGTCGACGGGGACATCGGAGGTTCGCATACTTTTCGTGTTCGATCCT
CGACGCTGCGCGGCCCTCCTTGTCGCGGGAAACAAGGAAGGGCAGTGGGAAAAATGGTATCGAAAAGCGATACCACTAGC
TGAAGAACGATACGAGGCGCATACCGCCGCAGCGGAGGTCGACGATGGCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10430.94 Da
• Isoelectric Point: 5.6558

>AT141521 WP_007924096.1 NZ_CP045221:c5191929-5191636 [Pseudomonas chlororaphis subsp. aurantiaca]
MNEPLFDYDPATALRDPQAIALFMADAFETGDAGYIAKALGVVARAKGMTELAHETGLSREQLYRSFSDKGNPTLKTLLA
VMRALGLDMTARVHGAS

Nucleotide

• Length: 294 bp

>AT141521 NZ_CP059997:c3767409-3767047 [Amycolatopsis sp. CA-230715]
ATGGCTGATTGGAAGGACGTAAAGGCGCGAAAGCGCGAGATCGATCAGGAAGCTGACCGTGACCTGCAGGCGGCGCGGTC
CGAAGCCAGTGCGCGTACTCACGCATACGTACTTGGGTACCGGTTGAGCGAATTGCGCGAACGCGTAGGGCTGAGCCAGA
CGCAGGTCGCTGAACGCATGGGGGTCAAGCAACCGCGCGTGAGTGCCATCGAAAAGGGTGACCCCGCCCAGATGGAGGTC
GAAACCCTGCAGCGCTACATCGCTGCTCTGGGCGGAAGATTGCGCGTGGTCGCCGGGTTTGGTGATCACGAAGAGATCGT
GTCGATCAGCGCGGTCGATCGGGACGAGTACGCATCGGCTTGA