Detailed information of TA system TA141519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 1619545..1620130
• Replicon: chromosome
• Accession: NZ_CP045221
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain ARS 38

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A7S5HMS4
• Locus tag: FD951_RS07575
• Protein ID: WP_016705075.1
• Coordinates: 1619837..1620130 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: FD951_RS07570
• Protein ID: WP_023968688.1
• Coordinates: 1619545..1619835 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FD951_RS07560	1617057..1618439	+	1383	WP_023968686.1	efflux transporter outer membrane subunit	-
FD951_RS07565	1618558..1619145	+	588	WP_023968687.1	hypothetical protein	-
FD951_RS07570	1619545..1619835	-	291	WP_023968688.1	putative addiction module antidote protein	Antitoxin
FD951_RS07575	1619837..1620130	-	294	WP_016705075.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FD951_RS07580	1620366..1621760	-	1395	WP_023968689.1	VOC family protein	-
FD951_RS07585	1622024..1623148	+	1125	WP_051408723.1	diguanylate cyclase	-
FD951_RS07590	1623242..1624486	+	1245	WP_023968691.1	M20/M25/M40 family metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-97)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10699.27 Da
• Isoelectric Point: 10.0959

>T141519 WP_016705075.1 NZ_CP045221:c1620130-1619837 [Pseudomonas chlororaphis subsp. aurantiaca]
MDYEILQTTVFAQWHTTLRDLRARIAIARRIERASAGNLGDVKNLGGGLSEMRVNTGAGYRLYFTVRGNTLIVLLLGGDK
SSQPADICQARSLAKEF

Nucleotide

• Length: 294 bp

>T141519 NZ_CP059997:2731237-2731479 [Amycolatopsis sp. CA-230715]
ATGAGCTACTCGGAGTCCCGCGCCAACTACGCGGAGACTCTTGACTCGGTGGTGAATGACCGCGAGGAAGTAGTGATCAC
CCGCGCTGGTCACGACCCCGTGGTGATCGTGTCGCTCGACGACTACGAATCACTGAAGGAAACGGCGTACCTGCTCCGCA
GCCCGGAGAACGCCCGGCGTCTCGTCTCCGCCATCGAACGGCTGGAGTCGGGTGGCGGCGAGCAGCACGAACTCGCCGAA
TGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10223.78 Da
• Isoelectric Point: 8.0096

>AT141519 WP_023968688.1 NZ_CP045221:c1619835-1619545 [Pseudomonas chlororaphis subsp. aurantiaca]
MSDTLLPFDIAALLDSDQAISEYLSQVLADGDTDELIRALGHVARARGMTQIAKTTGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCATVSRG

Nucleotide

• Length: 291 bp

>AT141519 NZ_CP059997:2731476-2731736 [Amycolatopsis sp. CA-230715]
ATGAAGCTCGTCTGGGACGCGTTCGCCTGGGACGACTACCTCTGGCGGCAAACCCAGGATCGCAAGGTCCTCAAGCGGAT
CAACGACCTCCTGAAGGACGTCCGGCGCAACGGGAACGAAGGCATCGGCAAGCCCGAGCCGCTGAAACACGGTTTCCAGG
GCTACTGGTCCCGTCGGATCACTGACGAGCACCGACTGGTCTACAAGATCGAAGGCGACGAAATTCGCATCGCGGCTTGC
CGATACCACTACGGCAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7S5HMS4