Detailed information of TA system TA141518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 12020..12756
• Replicon: plasmid unnamed1
• Accession: NZ_CP045217
• Organism: Pantoea dispersa strain BJQ0007

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: GAY20_RS20580
• Protein ID: WP_152391583.1
• Coordinates: 12274..12756 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: GAY20_RS20575
• Protein ID: WP_003026799.1
• Coordinates: 12020..12286 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GAY20_RS20535	7201..7431	+	231	WP_001568051.1	hypothetical protein	-
GAY20_RS20540	7483..8838	+	1356	WP_152391582.1	DUF3560 domain-containing protein	-
GAY20_RS20545	8886..9449	+	564	WP_130979841.1	methyltransferase	-
GAY20_RS20560	10260..11228	-	969	WP_013815101.1	IS5 family transposase	-
GAY20_RS20570	11347..11781	+	435	WP_032416767.1	cell envelope integrity protein TolA	-
GAY20_RS20575	12020..12286	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
GAY20_RS20580	12274..12756	+	483	WP_152391583.1	GNAT family N-acetyltransferase	Toxin
GAY20_RS20585	12980..13777	+	798	WP_193718043.1	hypothetical protein	-
GAY20_RS20590	13826..14230	+	405	WP_152391584.1	helix-turn-helix domain-containing protein	-
GAY20_RS20595	14628..14842	-	215	Protein_18	hypothetical protein	-
GAY20_RS20605	15500..16468	+	969	WP_013815101.1	IS5 family transposase	-
GAY20_RS20610	16672..17007	+	336	WP_032416808.1	TonB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..85113	85113
	-	inside	IScluster/Tn	-	-	10260..20933	10673

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17226.93 Da
• Isoelectric Point: 9.1886

>T141518 WP_152391583.1 NZ_CP045217:12274-12756 [Pantoea dispersa]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDISLHGKGVGADLLHDAVLRCYRVAENIGVSAIMVHALTEEAKGFYVHHGFKASQTHKRTLFLKLP

Nucleotide

• Length: 483 bp

>T141518 NZ_CP059996:31550-31804 [Sulfurospirillum sp. ACSDCE]
ATGAGTAAAAGAGATAAACTTTGGATCAAATTTATGGAAGTTCCACCGAAAAAGAATTTGACGTGGAGTGACTTATTGGC
ACTTATGTCAGCTCTTGATTTTGTGCAGCTTGAGGGTGATGGTTCACGTGTAAAGTTTTATCATCACGCTAAAGATATTG
TTCTCAATCTTCATAAACCACATCCAGATAATTTGCTCAAAACATATCTTGTAAAACAGATACAAGAAAAACTAAAGGAG
GCATTTAATGGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT141518 WP_003026799.1 NZ_CP045217:12020-12286 [Pantoea dispersa]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT141518 NZ_CP059996:31797-32141 [Sulfurospirillum sp. ACSDCE]
ATGGCTAATGTATTAACGTATGAAAATTATACAGGCTCGGTTGAATACAGTCATGAAGACAGGTGTTTCTTTGGAAAAAT
TGAATTTATTAATGATCTCATTACATTTGAAGCCACAACAGTGGATGAATTGGAGGCCAATTTCAAAGAGGCCGTTGAGA
GTTATATCCTTACGTGTAAAGCGTTAAATAGAAAACCACAAAAGCAATTTAATGGGGTCTTTAATGTCCGCCCTGGTGTT
GAACTTCATATGGCTGCAGCACGTAAAGCTATGGAGATTGGAGTTTCACTCAATGCATATATTAAAAGCCTTATCGCTAA
AGATACCCATTTTTCTGCACATTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66