Detailed information of TA system TA141516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3302326..3302946
• Replicon: chromosome
• Accession: NZ_CP045216
• Organism: Pantoea dispersa strain BJQ0007

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A246NIG6
• Locus tag: GAY20_RS15745
• Protein ID: WP_021507203.1
• Coordinates: 3302728..3302946 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: GAY20_RS15740
• Protein ID: WP_152391286.1
• Coordinates: 3302326..3302703 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GAY20_RS15710	3298649..3298906	+	258	WP_021507208.1	type B 50S ribosomal protein L31	-
GAY20_RS15715	3298922..3299062	+	141	WP_010616859.1	type B 50S ribosomal protein L36	-
GAY20_RS15720	3299107..3299985	-	879	WP_058769397.1	metal ABC transporter substrate-binding protein	-
GAY20_RS15725	3300007..3300846	-	840	WP_058775239.1	metal ABC transporter permease	-
GAY20_RS15730	3300843..3301499	-	657	WP_021507205.1	ABC transporter ATP-binding protein	-
GAY20_RS15735	3301827..3302180	+	354	WP_031279656.1	hypothetical protein	-
GAY20_RS15740	3302326..3302703	+	378	WP_152391286.1	Hha toxicity modulator TomB	Antitoxin
GAY20_RS15745	3302728..3302946	+	219	WP_021507203.1	hemolysin expression modulator Hha	Toxin
GAY20_RS15760	3303344..3304312	+	969	WP_013815101.1	IS5 family transposase	-
GAY20_RS15765	3304376..3305299	+	924	Protein_3030	IS5 family transposase	-
GAY20_RS15770	3305629..3306186	-	558	WP_021507201.1	YbaY family lipoprotein	-
GAY20_RS15775	3306390..3307253	+	864	WP_088516247.1	acyl-CoA thioesterase II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8538.87 Da
• Isoelectric Point: 8.8662

>T141516 WP_021507203.1 NZ_CP045216:3302728-3302946 [Pantoea dispersa]
MSNPALTKTDYLMRLRRCRSIDTLERVIEKNKYELPDDELAVFYSAADHRLAELTMNKLYDKVPGSVWKYVR

Nucleotide

• Length: 219 bp

>T141516 NZ_CP059995:c5739311-5739012 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 14664.36 Da
• Isoelectric Point: 4.5785

>AT141516 WP_152391286.1 NZ_CP045216:3302326-3302703 [Pantoea dispersa]
MDEYSPKRHDIAQLKFLCENLFDESMATLTDSHHGWVNDPTSPGNLQLNDLIEHIASFTMNYKIKHVEDEALITQIDEYL
NDTFMLFSSYGINTQDLQRWQRSAKRLFNLFAEECAYLQQPSHSF

Nucleotide

• Length: 378 bp

>AT141516 NZ_CP059995:c5739015-5738725 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCGACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGCGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: A0A246NIG6