Detailed information of TA system TA141515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2722806..2723420
• Replicon: chromosome
• Accession: NZ_CP045216
• Organism: Pantoea dispersa strain BJQ0007

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GAY20_RS12865
• Protein ID: WP_010617212.1
• Coordinates: 2722806..2722988 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GAY20_RS12870
• Protein ID: WP_022548759.1
• Coordinates: 2723028..2723420 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GAY20_RS12815	2717949..2718611	+	663	WP_021506360.1	MBL fold metallo-hydrolase	-
GAY20_RS12820	2718612..2719025	-	414	WP_058757613.1	GNAT family N-acetyltransferase	-
GAY20_RS12825	2719047..2719430	-	384	WP_152391104.1	hypothetical protein	-
GAY20_RS12830	2719650..2720006	+	357	WP_010617204.1	DUF4186 domain-containing protein	-
GAY20_RS12835	2720025..2720204	-	180	WP_031279245.1	hypothetical protein	-
GAY20_RS12840	2720408..2720737	+	330	WP_021506365.1	hypothetical protein	-
GAY20_RS12845	2720799..2721347	+	549	WP_058769856.1	hypothetical protein	-
GAY20_RS12850	2721372..2721578	-	207	WP_021506367.1	DUF2767 family protein	-
GAY20_RS12855	2721759..2722007	+	249	WP_021506368.1	DUF883 family protein	-
GAY20_RS12860	2722340..2722537	-	198	WP_021506369.1	hypothetical protein	-
GAY20_RS12865	2722806..2722988	+	183	WP_010617212.1	type II toxin-antitoxin system HicA family toxin	Toxin
GAY20_RS12870	2723028..2723420	+	393	WP_022548759.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GAY20_RS12875	2723505..2724761	+	1257	WP_021506372.1	mechanosensitive ion channel	-
GAY20_RS12880	2724883..2726133	-	1251	WP_010617214.1	NADP-dependent isocitrate dehydrogenase	-
GAY20_RS12885	2726235..2726903	+	669	WP_072208614.1	23S rRNA pseudouridine(2457) synthase RluE	-
GAY20_RS12890	2726948..2727421	+	474	WP_152391105.1	NUDIX hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-124)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6985.25 Da
• Isoelectric Point: 11.0007

>T141515 WP_010617212.1 NZ_CP045216:2722806-2722988 [Pantoea dispersa]
MKSATLIKLLQKNGWKLERIRGSHHQFSHPDFAHLITVPHPQKDMKKGTLMQILKDARLD

Nucleotide

• Length: 183 bp

>T141515 NZ_CP059995:c5739015-5738725 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCGACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGCGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 14554.49 Da
• Isoelectric Point: 4.8732

>AT141515 WP_022548759.1 NZ_CP045216:2723028-2723420 [Pantoea dispersa]
MLFPALVEIDADGSASGYFPDVTGCYFAGDTPEQTLQDAQSALHAHFELMAEKGLLIPEPAQHWQQEFSQPGVWIYVDID
VTRYLGKSERINITMPHLLIEKIDKMVSNNARYSSRSHFLAEAARKALVS

Nucleotide

• Length: 393 bp

>AT141515 NZ_CP059995:c5739311-5739012 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA