Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 102603..103128 Replicon plasmid pO113-EH41
Accession NZ_CP045214
Organism Escherichia coli strain EH41

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag GC231_RS25195 Protein ID WP_001159871.1
Coordinates 102603..102908 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag GC231_RS25200 Protein ID WP_000813639.1
Coordinates 102910..103128 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC231_RS25165 (GC231_25470) 98598..98771 + 174 Protein_107 DUF4113 domain-containing protein -
GC231_RS25170 (GC231_25475) 98773..99189 - 417 WP_001278809.1 plasmid partitioning/stability family protein -
GC231_RS25175 (GC231_25480) 99182..100162 - 981 WP_000688508.1 plasmid segregation protein ParM -
GC231_RS25180 (GC231_25485) 100577..100885 - 309 WP_000030199.1 hypothetical protein -
GC231_RS25185 (GC231_25490) 100972..101616 - 645 WP_001144036.1 ParA family protein -
GC231_RS25190 (GC231_25495) 101796..102602 - 807 WP_000016966.1 site-specific integrase -
GC231_RS25195 (GC231_25500) 102603..102908 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
GC231_RS25200 (GC231_25505) 102910..103128 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
GC231_RS25205 (GC231_25510) 103724..103984 + 261 WP_000343100.1 hypothetical protein -
GC231_RS25210 (GC231_25515) 103981..104571 + 591 WP_000194569.1 hypothetical protein -
GC231_RS25215 (GC231_25520) 104589..104936 - 348 WP_000142427.1 DUF6404 family protein -
GC231_RS25220 (GC231_25525) 105419..105790 - 372 WP_001373114.1 colicin-like pore-forming protein -
GC231_RS25225 (GC231_25530) 105850..106581 - 732 WP_000850414.1 conjugal transfer complement resistance protein TraT -
GC231_RS25230 (GC231_25540) 106853..106963 - 111 Protein_120 IS481 family transposase -
GC231_RS25235 (GC231_25545) 106967..107184 - 218 Protein_121 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyA / hlyC / espP / espP 1..169291 169291


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T141510 WP_001159871.1 NZ_CP045214:c102908-102603 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T141510 NZ_CP059995:1993717-1994304 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAATTCCTTCCCGCATTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT141510 WP_000813639.1 NZ_CP045214:c103128-102910 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT141510 NZ_CP059995:1994371-1995225 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACACACGCATTTCTGGCGCGCAGCCGAACTGGGCGGCGTCGAACTGCTGCACGCGCG
CTATGTGGAGCAGCGCTTCGCGCCGCATGTGCACAGCAGCTTCGCCCTGGTCATCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGTGGCGGCGAGCACCTGGCCCCGCTGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGATGCCGGTTGGCTCTATCGCGGCTACTACCCGGAAATGGAGCAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTGCCGCGTTTCGCCGACAGCGTGATCCATGACCAGCCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGGCAGCCTGGAAACCCAGGCCCGCTGGCGCGAGACGATGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCAGAAGCGCCGAAGGCCGGGCAGGAGCCGTTGGCGGTGGCTCGCGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGATCCGCCTTCACTGGAGGAACTGGCGGCGGCGGTCAATCTATCCCCGTTCCATTTTGCCCGGGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGGCAGCGCCGGCTGGAGCAGGCCAGGGCACTGCTCAAGAGCGGCCGGGCG
CCGTTGGAGGTGGCTCTGGAGCTGGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGACCTACGGCGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCGTCCTTGACGACATACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References