Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4726771..4727357 Replicon chromosome
Accession NZ_CP045213
Organism Escherichia coli strain EH41

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag GC231_RS23230 Protein ID WP_042014162.1
Coordinates 4727127..4727357 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag GC231_RS23225 Protein ID WP_000593555.1
Coordinates 4726771..4727130 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC231_RS23200 (4722903) 4722903..4723847 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
GC231_RS23205 (4723844) 4723844..4724677 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
GC231_RS23210 (4724677) 4724677..4725441 + 765 WP_228400279.1 nickel import ATP-binding protein NikD -
GC231_RS23215 (4725438) 4725438..4726244 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
GC231_RS23220 (4726250) 4726250..4726651 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
GC231_RS23225 (4726771) 4726771..4727130 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GC231_RS23230 (4727127) 4727127..4727357 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
GC231_RS23235 (4727461) 4727461..4728585 - 1125 WP_001314210.1 ABC transporter permease -
GC231_RS23240 (4728585) 4728585..4731320 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T141509 WP_042014162.1 NZ_CP045213:c4727357-4727127 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T141509 NZ_CP059995:c144431-144150 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT141509 WP_000593555.1 NZ_CP045213:c4727130-4726771 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT141509 NZ_CP059995:c144655-144428 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References