Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4705264..4706064 Replicon chromosome
Accession NZ_CP045213
Organism Escherichia coli strain EH41

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag GC231_RS23105 Protein ID WP_000342449.1
Coordinates 4705264..4705791 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag GC231_RS23110 Protein ID WP_001277108.1
Coordinates 4705798..4706064 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC231_RS23080 (4700339) 4700339..4701106 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
GC231_RS23085 (4701103) 4701103..4702380 - 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
GC231_RS23090 (4702377) 4702377..4703303 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
GC231_RS23095 (4703351) 4703351..4704460 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
GC231_RS23100 (4704884) 4704884..4705267 + 384 WP_220401121.1 aspartate 1-decarboxylase autocleavage activator PanM -
GC231_RS23105 (4705264) 4705264..4705791 - 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
GC231_RS23110 (4705798) 4705798..4706064 - 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
GC231_RS23115 (4706214) 4706214..4707317 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
GC231_RS23120 (4707588) 4707588..4708442 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
GC231_RS23125 (4708687) 4708687..4709745 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
GC231_RS23130 (4709738) 4709738..4710406 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T141508 WP_000342449.1 NZ_CP045213:c4705791-4705264 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T141508 NZ_CP059993:76415-76741 [Xanthomonas citri]
ATGATGCGGGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAAAACCAAGGCCCGCTCTGGTGATCCAGGCCGA
CCAGTTTGACGCACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTCGTTGCTGCGCCGCTGCTGCGTATTACCG
TTCATCCAAGTACCGACAATGGTTTGCAGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
GTAGGTCGAGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT141508 WP_001277108.1 NZ_CP045213:c4706064-4705798 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT141508 NZ_CP059993:76248-76418 [Xanthomonas citri]
ATGGCAGGGCTGCGTCCGGTGCAAATTTGGGTGCCGGACACACGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTG
TCGTCTTGCTGCACAAGCGGACATGGCGGATACCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCT
GGACGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References