Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4042253..4042907 Replicon chromosome
Accession NZ_CP045213
Organism Escherichia coli strain EH41

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag GC231_RS19800 Protein ID WP_000244772.1
Coordinates 4042253..4042660 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GC231_RS19805 Protein ID WP_000354046.1
Coordinates 4042641..4042907 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC231_RS19780 (4038210) 4038210..4039943 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
GC231_RS19785 (4039949) 4039949..4040659 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GC231_RS19790 (4040684) 4040684..4041580 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GC231_RS19795 (4041692) 4041692..4042213 + 522 WP_001055874.1 flavodoxin FldB -
GC231_RS19800 (4042253) 4042253..4042660 - 408 WP_000244772.1 protein YgfX Toxin
GC231_RS19805 (4042641) 4042641..4042907 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GC231_RS19810 (4043150) 4043150..4044130 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
GC231_RS19815 (4044207) 4044207..4044866 - 660 WP_000250274.1 hemolysin III family protein -
GC231_RS19820 (4045030) 4045030..4045341 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
GC231_RS19825 (4045386) 4045386..4046819 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
GC231_RS19830 (4046876) 4046876..4047619 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T141505 WP_000244772.1 NZ_CP045213:c4042660-4042253 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T141505 NZ_CP059992:5120379-5120795 [Xanthomonas citri]
GTGATCCTGCTCGATACCAATGTCATCTCCGAACTTTGGCGGCCTCAGCCTAATCCGCAGGTCGTCGCCTGGATCGATGC
ACAAGCTGTCGAGACACTGTTCCTGTCGGTCGTGACGGTGGCTGAACTCCGTTTCGGTATTGCCGTGATGCCCAAGGGCC
GAAAGCGATCCACCTTGCATGCTCGGTTGGAGGGCGAGGTATTGCCACTGTTCGACGGGCGCCTGCTCGCATTCGATCTG
GATGCATCACACGCATTTGCGGCGCTTGCATCGAAAGCCAGAACCGCAGGACTCACACTCGGGCGCGCCGATGCCTACAT
CGCTGCAACAGCAGCCGCGCAAGGATTGACCGTTGCCACTCGCGACACGGCACCATTCGAGGCGATGGCGTTAGACGTCA
TTAACCCTTGGAGTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT141505 WP_000354046.1 NZ_CP045213:c4042907-4042641 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT141505 NZ_CP059992:5120128-5120382 [Xanthomonas citri]
ATGCCTTCTTTCACCGTCCGCAATATCCCAGACGACGTGCATCGCGCGATCCGCGCGCGGGCTGCACTTCATGGCCGCAG
CACCGAAGCGGAGATCCGCGCAATCCTTGAATTGGCGGCCAAACCCGCAGACCGGGTCAAACTCGGCTCACTGCTGGTGA
GCATCGGCCGCGACGCCGGCCTGACCGCAAAAGAGGCCAATGCCTTCGACAAGCTGCGCGGCAAGGCGGCGATCAAGCCG
ATCGGTCTGAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References