141504

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3905990..3906573 Replicon chromosome
Accession NZ_CP045213
Organism Escherichia coli strain EH41

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag GC231_RS19185 Protein ID WP_000254745.1
Coordinates 3905990..3906325 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag GC231_RS19190 Protein ID WP_000581937.1
Coordinates 3906325..3906573 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC231_RS19165 (3901459) 3901459..3901821 + 363 WP_063075011.1 hypothetical protein -
GC231_RS19170 (3901877) 3901877..3903175 - 1299 WP_000036723.1 phosphopyruvate hydratase -
GC231_RS19175 (3903263) 3903263..3904900 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
GC231_RS19180 (3905128) 3905128..3905919 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
GC231_RS19185 (3905990) 3905990..3906325 - 336 WP_000254745.1 endoribonuclease MazF Toxin
GC231_RS19190 (3906325) 3906325..3906573 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
GC231_RS19195 (3906651) 3906651..3908885 - 2235 WP_000226815.1 GTP pyrophosphokinase -
GC231_RS19200 (3908933) 3908933..3910234 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T141504 WP_000254745.1 NZ_CP045213:c3906325-3905990 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T141504 NZ_CP059992:c4721609-4721457 [Xanthomonas citri]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCCGGCGC
CGGCAAGGACATGCAGGGTGCGGGCAACAAGGTCGAGAAAACGGCCGAAAAATGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT141504 WP_000581937.1 NZ_CP045213:c3906573-3906325 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT141504 NZ_CP059992:c4721382-4721248 [Xanthomonas citri]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References