Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2602057..2602583 Replicon chromosome
Accession NZ_CP045213
Organism Escherichia coli strain EH41

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag GC231_RS12730 Protein ID WP_000323025.1
Coordinates 2602057..2602344 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag GC231_RS12735 Protein ID WP_000534858.1
Coordinates 2602344..2602583 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC231_RS12680 (2597081) 2597081..2597296 - 216 WP_000839590.1 phage lysis protein EssD -
GC231_RS12685 (2597516) 2597516..2597686 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
GC231_RS12690 (2598050) 2598050..2598265 - 216 WP_000066484.1 cold shock-like protein CspB -
GC231_RS12695 (2598566) 2598566..2598778 + 213 WP_000087756.1 cold shock-like protein CspF -
GC231_RS12700 (2598833) 2598833..2598922 + 90 WP_120795389.1 hypothetical protein -
GC231_RS12705 (2599200) 2599200..2599952 - 753 WP_001047135.1 antitermination protein -
GC231_RS12710 (2599966) 2599966..2601015 - 1050 WP_001265198.1 DUF968 domain-containing protein -
GC231_RS12715 (2601017) 2601017..2601295 - 279 WP_012304870.1 hypothetical protein -
GC231_RS12720 (2601362) 2601362..2601613 - 252 WP_000980994.1 protein Rem -
GC231_RS12725 (2601830) 2601830..2601985 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
GC231_RS12730 (2602057) 2602057..2602344 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
GC231_RS12735 (2602344) 2602344..2602583 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
GC231_RS12740 (2602608) 2602608..2602913 + 306 WP_001326990.1 protein YdfV -
GC231_RS12745 (2603116) 2603116..2603448 + 333 WP_001317460.1 FlxA-like family protein -
GC231_RS12750 (2603885) 2603885..2604034 - 150 WP_011443592.1 protein YdfW -
GC231_RS12755 (2604107) 2604107..2605177 - 1071 Protein_2480 ISNCY family transposase -
GC231_RS12760 (2605968) 2605968..2606255 - 288 Protein_2481 hypothetical protein -
GC231_RS12765 (2606733) 2606733..2607222 - 490 Protein_2482 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2566285..2615831 49546


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T141493 WP_000323025.1 NZ_CP045213:c2602344-2602057 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T141493 NZ_CP059963:c4732200-4730881 [Pectobacterium brasiliense]
ATGGCTGCTCTCGATGTTTACATGAACGGCTATCGGGTGGGGATTCTGACCAAAACGGGCAGTGGGGCACATCATTTTTC
CTATGATACGAACTGGCTCGGATTGCTGGGGAGTCGCCCCATTTCGCTTTCTATGCCGCTACGTCATCAGCCCTATCAAG
GCGATGAAGTTTATAACTTCTTCGACAATTTGTTGCCGGATAACCCGGATATCAGAAGACGGATTGTCGCCAGGCATCAT
ACGGATTCCACCCAACCGTTTGATCTGCTCGCTAAAGTGGGGCAAGACAGTGTCGGGGCGTTGCAATTAGTGCCGCAGGG
TACACCTGTGCACGATATAAAAAAGATCGAATATAAAACCTTATCCGAGCAGCAGCTTGAAAACATACTGACCGGATATT
TGTCCGATGCCCCGCTGGGTATGATTGATACTGAAGACGATTTTCGTATTTCTATCGCAGGTGCACAGGAAAAGACGGCA
TTGCTTTATCTGGATGATCGCTGGTGCCTGCCTCTCAATGTCACACCAACTACCCACATCATCAAGCTACCGATTGGTAA
AATCGAGAGTCACTCTTACTCAATTGATATGTCTGACAGCGTTGAAAATGAGTATCTGTGTTTGCTCATTGCCAAGGCAT
TTGGGCTGCCAGTGCCGCACTGTTTTATGATCAACGCTGGCAAGATAAAGGCGTTAGCGGTGGAGCGTTTTGATCGTAAA
TACGCCTCGGATGTCAGTTGGATTATGCGGCTACCACAAGAGGATTTTTGTCAGGTATTAAACGTTCCTTCCGCACTGAA
ATATGAAAATCATGGTGGACCGGGTATTTCCGCCATTATGTCGTTTCTATTGGGTTCGGTTTATCCAGAAAGAGATCGTT
ATCGCTTTATGCAGACTCAGGTACTGTTTTGGCTATTGGCTGCAACGGATGGTCATGCGAAAAACTTTTCCCTGTTTATT
GAATCTGAGGGACGATATCGACTCACGCCTTTCTATGACATTCTCTCTATGTATCCGGCGATGGGCGGACGGGGAATTGA
CCGCAGAGAGGCCAAACTGGCGATGGGATTAACTGGCTCAAGAGGCAAGAAATATGCGATTGAGCAGATCTTTCCCCGAC
ATTTTTTCCAGACGGCCAAGGTCGTTGGGTTCGCCAGAGAATCGATGGAAAACATTCTGGCGGAGTTTGCCCAGACCGTT
GATACCGTCATGACGACTGTTGGAAGTCAACTGCCTGTGGATTTTCCTACTCATATCAGTGAGGCAATTCTGGCCGGATT
GCAGGCCAGATCGAGACGGCTCATGAGCGGATGGGAGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT141493 WP_000534858.1 NZ_CP045213:c2602583-2602344 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT141493 NZ_CP059963:c4732469-4732200 [Pectobacterium brasiliense]
ATGATGAAGATCACCAACAGCAAGCAGCTTAGCAGCTATCTGAAAGATGTTCGTATCACGCAGAAGCTTTCACAAGGGAA
AGTGGCCAGTAAAGTGGGTATTCGCCAGGATACGGTATCCAGTTTCGAGCAACATCCTGATTCCACTAAGTTGGAAACCT
TCTTTAAAATCCTGTCGGCATTGAATTTAGAACTTACTGTTACGCCCAGGAATGTGGACACCACCAATAACGAAGCCTCT
GTTGCATCATCCTGGAAGGAAGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References