Detailed information of TA system TA141492

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2519402..2520990
• Replicon: chromosome
• Accession: NZ_CP045213
• Organism: Escherichia coli strain EH41

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U9XU38
• Locus tag: GC231_RS12280
• Protein ID: WP_001125455.1
• Coordinates: 2519402..2520724 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GC231_RS12285
• Protein ID: WP_001296726.1
• Coordinates: 2520724..2520990 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GC231_RS12265 (2516814)	2516814..2517524	-	711	WP_001195166.1	fimbria/pilus periplasmic chaperone	-
GC231_RS12270 (2517885)	2517885..2518448	-	564	WP_001296758.1	type 1 fimbrial major subunit FimA	-
GC231_RS12275 (2518882)	2518882..2519090	-	209	Protein_2384	AraC family transcriptional regulator	-
GC231_RS12280 (2519402)	2519402..2520724	-	1323	WP_001125455.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
GC231_RS12285 (2520724)	2520724..2520990	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49321.93 Da
• Isoelectric Point: 8.8753

>T141492 WP_001125455.1 NZ_CP045213:c2520724-2519402 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T141492 NZ_CP059963:4700177-4700563 [Pectobacterium brasiliense]
ATGGAGGTTTACAGATTGGCATGGGAAATAGTCACACGGGCACTATTCGATACGTGGTTTGATGAGCAAACTGAAACGGT
ACAGGAAGAGGTTTTGGCACACCTCGCTATACTCGAAGAGGATGGCCCACAGCTAGGGCGACCACAGGTTGATCAGATTA
AAGGCTCAAAATACAAAAATATGAAGGAGCTTCGAGTTCAGGTTGGCGGCCACCCATTCCGAACATTCTTCGCGTTTGAC
CGGACACGTAAAGCGATTGTTTTATGTGCGGGAGATAAGAAAGGCGAAAATGCAAAGTTGTTCTACGAACGTATGATCAA
GATCGCAGACGCTGAGTTTGCATCGCATCTGATATCACCGGAGGTAAAAGAAGATGGCGACGCTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT141492 WP_001296726.1 NZ_CP045213:c2520990-2520724 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT141492 NZ_CP059963:4700550-4700849 [Pectobacterium brasiliense]
ATGGCGACGCTTAAAGAAATGATGGCTAAACGTTCACCGGAGAGCCGCGCCCGCATTGAAGCGCGCACGGCTGAGATCCG
TCAGGAAATCACTTTAGCGAAACTACGCGAAGAGCTGAACATGTCGCAGACTCAGCTTGCGTTAGCATTGGGCGTCAAGC
AGCCATCTATTGTTAAGATGGAGAAGGTCGAAAATGACCCAAAACTGTCAACGCTGAAACGCTATGTCACTGCCCTAGGC
GGCAAGCTTACGCTAGATGTGACCTTGCCTGATGGTAAGCGTATCGCGCTTACTTTGTAA

Structures

Toxin

• AlphaFold DB: U9XU38