Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1309731..1310349 Replicon chromosome
Accession NZ_CP045213
Organism Escherichia coli strain EH41

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GC231_RS06250 Protein ID WP_001291435.1
Coordinates 1309731..1309949 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GC231_RS06255 Protein ID WP_000344800.1
Coordinates 1309975..1310349 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC231_RS06215 (1305020) 1305020..1305592 + 573 WP_000779831.1 YbaY family lipoprotein -
GC231_RS06220 (1305623) 1305623..1305934 - 312 WP_000409911.1 MGMT family protein -
GC231_RS06230 (1306313) 1306313..1306666 + 354 WP_000878140.1 DUF1428 domain-containing protein -
GC231_RS06235 (1306708) 1306708..1308258 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GC231_RS06240 (1308422) 1308422..1308892 - 471 WP_000136192.1 YlaC family protein -
GC231_RS06245 (1309008) 1309008..1309559 - 552 WP_000102568.1 maltose O-acetyltransferase -
GC231_RS06250 (1309731) 1309731..1309949 - 219 WP_001291435.1 HHA domain-containing protein Toxin
GC231_RS06255 (1309975) 1309975..1310349 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GC231_RS06260 (1310895) 1310895..1314044 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
GC231_RS06265 (1314067) 1314067..1315260 - 1194 WP_044862765.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T141490 WP_001291435.1 NZ_CP045213:c1309949-1309731 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T141490 NZ_CP059963:c4566485-4566210 [Pectobacterium brasiliense]
ATGCAGGGAAAAATAGCCAGCCTACGAAAGAAGCAGCGGGAAACGCTGATCCAAATTTTCAAAACACCAGTACTTTCAGG
TGTTAAATGGTCGAATATTGAGTCACTCGTTACTGCGTTAGGCGGTGAAATTAAGGAAGGTAGCGGATCGCGAGTGCGAT
TTTTATTGAACGGGAGTATCGCCCGGTTCCATCGTCCCCATCCGTCACCGGATACTGATAAAGGTGCACTGGTTAGCCTG
CGTGAATGGCTGGAAAGTATAGGAGTAAAACCATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT141490 WP_000344800.1 NZ_CP045213:c1310349-1309975 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT141490 NZ_CP059963:c4566213-4565851 [Pectobacterium brasiliense]
ATGACGAAAGCACTCAACACACCAAATACGATGGTTGTTTCTGGACAACCGGCCATTATCAGTTATGTACCGGAAATGGG
TATGTTTCGCGGTAAGTTTATTGGTTTATCTGGCTACTGTGATTTTGTCGCCGACAGTATTAATGGCTTGAGAAGCGAGG
GTGAAATTTCGCTACGCGAATATCTGGAAGACTGTCAGGAAAATGGCATTGAACCTTACGAGCGTGAAGAGAAGGTGAAA
ACATTCACCCTCCGCTACCCTGAATCATTTGGGGAACGACTGACTATTGCGGCTGCTGAGCGCCAAATTTCAGTGAATAC
GTTTATTGTTGAAACGCTTAGCGAACACCTGAAACAGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References