Detailed information of TA system TA141489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1275423..1276260
• Replicon: chromosome
• Accession: NZ_CP045213
• Organism: Escherichia coli strain EH41

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: GC231_RS06080
• Protein ID: WP_000227784.1
• Coordinates: 1275423..1275965 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: GC231_RS06085
• Protein ID: WP_001297137.1
• Coordinates: 1275949..1276260 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GC231_RS06060 (1270962)	1270962..1271873	-	912	WP_000705853.1	2-dehydropantoate 2-reductase	-
GC231_RS06065 (1272041)	1272041..1272532	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
GC231_RS06070 (1272660)	1272660..1274024	-	1365	WP_001000978.1	MFS transporter	-
GC231_RS06075 (1274432)	1274432..1275367	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
GC231_RS06080 (1275423)	1275423..1275965	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
GC231_RS06085 (1275949)	1275949..1276260	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
GC231_RS06090 (1276445)	1276445..1277335	-	891	WP_000971336.1	heme o synthase	-
GC231_RS06095 (1277347)	1277347..1277676	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
GC231_RS06100 (1277676)	1277676..1278290	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
GC231_RS06105 (1278280)	1278280..1280271	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
GC231_RS06110 (1280293)	1280293..1281240	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T141489 WP_000227784.1 NZ_CP045213:c1275965-1275423 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T141489 NZ_CP059963:4542054-4542233 [Pectobacterium brasiliense]
ATGGATAGCAGGACGTTAATAGCAGAAATCAAGGCCGATGGGTGGGAACTGATAAGAGTGAACGGAAGCCACCACCATTT
TATGCATCCTAGTAAACCGGGGTTAGTCACAATCCCCCACCCTAAGAAAGATTTACCGATAGGCACGGTAAAAAGCATCA
GGAAACAAGCAGGAATATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT141489 WP_001297137.1 NZ_CP045213:c1276260-1275949 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT141489 NZ_CP059963:4542261-4542671 [Pectobacterium brasiliense]
ATGTTTTATCCGATTGCAATTGAAGCAGGCGACGATACCCACGCATACGGTGTAACAGTGCCAGATCTACCGGGCTGTTT
TTCTGCAGGCGATACGCTAGACGATGCCATCGCGAATGCTAAAGAGGCGATTACCGGACATATCGAACTGCTGATCGAGA
TGGGTCAGGATATTCCCACCGTGTCTTCAGTAGGCCAATTGGCAAAAAGCGCAGAATACGCTGGGTACACGTGGGCGGTT
GTTGATATCGATGTGACGCGTTTAATGGGCGGTTCAGAAAAAATTAACGTCACGCTGCCTAAATCACTGATTGACCGAAT
TGATCGTTGCGTGGCGAGTAATCCTGAATTTAAAAGCCGCTCAGGTTTTCTCGCGCAAGCGGCATTAGAGCGAATCTCGT
CTTCCCGATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3