Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 709153..709985 Replicon chromosome
Accession NZ_CP045213
Organism Escherichia coli strain EH41

Toxin (Protein)


Gene name yeeV Uniprot ID S1PD64
Locus tag GC231_RS03455 Protein ID WP_000854753.1
Coordinates 709611..709985 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID V0ULY5
Locus tag GC231_RS03450 Protein ID WP_001315620.1
Coordinates 709153..709521 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC231_RS03420 (705989) 705989..706444 + 456 WP_000581504.1 IrmA family protein -
GC231_RS03425 (706523) 706523..706756 + 234 WP_001119729.1 DUF905 family protein -
GC231_RS03430 (706856) 706856..707674 + 819 WP_001234620.1 DUF932 domain-containing protein -
GC231_RS03435 (707729) 707729..708214 + 486 WP_000849588.1 antirestriction protein -
GC231_RS03440 (708230) 708230..708706 + 477 WP_220401182.1 RadC family protein -
GC231_RS03445 (708769) 708769..708990 + 222 WP_000692345.1 DUF987 domain-containing protein -
GC231_RS03450 (709153) 709153..709521 + 369 WP_001315620.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
GC231_RS03455 (709611) 709611..709985 + 375 WP_000854753.1 TA system toxin CbtA family protein Toxin
GC231_RS03460 (709982) 709982..710470 + 489 WP_000777547.1 DUF5983 family protein -
GC231_RS03465 (710482) 710482..710679 + 198 WP_000839281.1 DUF957 domain-containing protein -
GC231_RS03470 (710764) 710764..710913 + 150 Protein_660 restriction endonuclease subunit M -
GC231_RS03475 (711673) 711673..711816 - 144 Protein_661 HNH endonuclease -
GC231_RS03480 (711953) 711953..712603 + 651 WP_001037966.1 HNH endonuclease -
GC231_RS03485 (712611) 712611..713591 - 981 WP_001295601.1 sialate O-acetylesterase -
GC231_RS03490 (713656) 713656..714762 - 1107 WP_001315214.1 N-acetylneuraminate epimerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14005.00 Da        Isoelectric Point: 8.2905

>T141487 WP_000854753.1 NZ_CP045213:709611-709985 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Download         Length: 375 bp

>T141487 NZ_CP059963:3390823-3391167 [Pectobacterium brasiliense]
ATGAAATCACTCTACTGGGTTGGCAGTAGCAAAAAGGATCTCCAATCGCTACCTGCAGATGTGCAGGATGTGTTCGGTTA
CGCACTTCATGTGGCTCAGACTGGTGGTAAGCACACTCAAGCCAAGCCGCTTAAAGGTTTTGGTGGTGCAGGCGTATTAG
AAGTCGTTGAGGATTATATCGGCGATACTTATCGCGCAGTCTACACCGTCAAATTTGGAGAGGCAGTGTACGTTCTACAT
ACTTTTCAGAAAAAATCGTCATCTGGAATTGCAACACCGAAATCCGATATGAACACCATCCGTGAGCGCCTGAAAACCGC
AGAAAACCATGCAAAAGGAGCATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13474.20 Da        Isoelectric Point: 6.2050

>AT141487 WP_001315620.1 NZ_CP045213:709153-709521 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT141487 NZ_CP059963:3391169-3391486 [Pectobacterium brasiliense]
ATGAGCCTGGATATTGAAGTCAGCAGCGGTAATGTGTACGCCGACCTTGGCAACGTGCATGCTGAAGATATGTTGGTCAA
AGCGCAGCTTGCCACACTGATTAGCTCAATCATCAAAAGCCGCCGTCTCACACAGGCAGAAGCAGCGAAGCTATTGGGCA
TGCCCCAACCCAAACTATCCAACTTATTACGTGGTCAGTTCAGGGGAATCAGTGAAACCAAAATGTTGGAATGCCTCACG
AAGCTGGGACGCGATATACAGATCGTGGTAGGGAAGCCGCGCCGCACGCCGGGTAGCGTTAAAGTCGTGTTTGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LVU0

Antitoxin

Source ID Structure
AlphaFold DB V0ULY5

References