Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 481650..482147 Replicon chromosome
Accession NZ_CP045213
Organism Escherichia coli strain EH41

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag GC231_RS02325 Protein ID WP_001173343.1
Coordinates 481974..482147 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag GC231_RS02320 Protein ID WP_000398619.1
Coordinates 481650..481946 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC231_RS02300 (478381) 478381..479100 - 720 WP_000611288.1 two-component system response regulator DcuR -
GC231_RS02305 (479097) 479097..480728 - 1632 WP_001216461.1 sensor histidine kinase -
GC231_RS02310 (480909) 480909..481139 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
GC231_RS02315 (481151) 481151..481423 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
GC231_RS02320 (481650) 481650..481946 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GC231_RS02325 (481974) 481974..482147 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
GC231_RS02330 (482266) 482266..483783 - 1518 WP_001295074.1 lysine--tRNA ligase -
GC231_RS02335 (484020) 484020..485477 - 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T141486 WP_001173343.1 NZ_CP045213:481974-482147 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T141486 NZ_CP059963:1746346-1746660 [Pectobacterium brasiliense]
ATGCAATTCACTGTATACGGTAATACCGGGAAAAGCGTCGTTTACCCGCTGTTGCTCGATGTCACGAGCGATATCATTGG
GCAATTGAATCGTCGGATAATGATCCCATTGCTCCCAATTGAAAAGTATCCGGCAGGCCGCCGACCAGATCGTCTTGTCC
CCGTCGTCAGGCTGACGGACGGCAAAGAATACGCCGTAATGACCCACGAGCTGGCAAGTATCCCTGTCCAGGCCCTGGGT
GCGGTGTTTTGTGATGCTTCGCAGTACCGTTCTCAGGTAAAAGCTTCAATAGACTTCCTCATCGACGGGTTCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT141486 WP_000398619.1 NZ_CP045213:481650-481946 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT141486 NZ_CP059963:1746107-1746343 [Pectobacterium brasiliense]
ATGACCGCGAAACAACGCAACACGCAGAGCGTGACCATGACAGTAGAGCGTGCCTTACTGGTAAGAGCGCGTGAAGCGGG
CATCAATCTGAGTGCTACCCTGACAACCGCTCTGGATGCAGAGCTTCGCTCTCATGAAGCGAAAAAATGGCAGGAAGAAA
ACAGGGAGGCTCTCGCAGCACTCAATCGTTTTCATGATGAGCAGGGTTGTTTCAGCGATGAATACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References