Detailed information of TA system TA141481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 41413..41948
• Replicon: plasmid p65-4
• Accession: NZ_CP045211
• Organism: Escherichia coli strain 4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GC226_RS24835
• Protein ID: WP_001270419.1
• Coordinates: 41413..41700 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: GC226_RS24840
• Protein ID: WP_001132895.1
• Coordinates: 41697..41948 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GC226_RS24810 (37009)	37009..37380	-	372	WP_228400227.1	hypothetical protein	-
GC226_RS24815 (38210)	38210..38803	-	594	WP_000166787.1	tyrosine-type DNA invertase	-
GC226_RS24820 (39704)	39704..39814	+	111	WP_224068057.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GC226_RS24825 (40187)	40187..40852	+	666	Protein_36	tyrosine-type recombinase/integrase	-
GC226_RS24830 (41166)	41166..41411	-	246	Protein_37	integrase core domain-containing protein	-
GC226_RS24835 (41413)	41413..41700	-	288	WP_001270419.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GC226_RS24840 (41697)	41697..41948	-	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GC226_RS24845 (42336)	42336..43541	+	1206	WP_000817641.1	AAA family ATPase	-
GC226_RS24850 (43538)	43538..44500	+	963	WP_000756326.1	ParB family protein	-
GC226_RS24855 (44733)	44733..45512	+	780	Protein_42	IS4 family transposase	-
GC226_RS24860 (45643)	45643..45726	-	84	Protein_43	DUF4113 domain-containing protein	-
GC226_RS24865 (45728)	45728..46256	+	529	Protein_44	DUF1281 domain-containing protein	-
GC226_RS24870 (46260)	46260..46565	+	306	WP_032163448.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..65782	65782

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.3527

>T141481 WP_001270419.1 NZ_CP045211:c41700-41413 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLHGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T141481 NZ_CP059963:354943-355437 [Pectobacterium brasiliense]
ATGATCTCCGCTCCTGAGCCACTCCGCGCCGAACATATACTATCCTCATTCTGTTGCGGCGTGGAGTCTATGGACAACTG
GCTGAAACAACGGGCGATGAAAAATCAGGTCACTGGCGCATCCCGAACTTTCGTCAGTTGCGATGATTCAAAAGTGTTGG
CGTATTACTCATTGGCTTCCAGCGCCGTGGCAACGAATGCCGCCCCCGGACGCTTTCGTCGCAATATGCCCGATCCGATC
CCGGTGGTAGTGCTGGGACGTCTGGCGGTTGATACGTCATTGCATGGTCAAGGTGTTGGACGGGCATTGGTAAGAGATGC
TGGGCTACGGGTGATTCAGGTTGCGGATACTATCGGTATTCGCGGGATGTTAGTTCATGCGCTGTCTGACGAAGCGCGGG
AGTTTTACCTACGAGTGGGGTTTGAGCCTTCGCCGATTGACCCGATGATGTTGATGGTGACGTTAGGGGATTTGGTGGGG
AGTTTATCAATTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT141481 WP_001132895.1 NZ_CP045211:c41948-41697 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT141481 NZ_CP059963:354653-354946 [Pectobacterium brasiliense]
ATGCCAGCGACGAATAGCGTTTCTGTTAAACGTGAAACATTGAATTTACGCATCAAGCCCGCCGAACGCGACCTTATCGA
CCGTGCGGCGAAGGCCAGAGGAAAAAATCGCACTGATTTTGTCCTCGAAGCTGCTCGTGCCGCTGCAGAGGAAGCGTTGA
TCGATCAGCGCATCATCATGGCAGATCCCGACGCTTATCAGGAATTCCTTGCCCGCCTGGATCAGGCTCCTGCACCAAAT
GCCGCGCTGCGAAAAACCATGCAAACGCCTGCACCGTGGGAACAGAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6