Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 25233..25807 Replicon plasmid p65-4
Accession NZ_CP045211
Organism Escherichia coli strain 4

Toxin (Protein)


Gene name relE Uniprot ID A0A0F6SKD8
Locus tag GC226_RS24760 Protein ID WP_000421260.1
Coordinates 25517..25807 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag GC226_RS24755 Protein ID WP_001178089.1
Coordinates 25233..25517 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC226_RS24725 (20953) 20953..21096 - 144 Protein_16 DNA-binding protein -
GC226_RS24730 (21160) 21160..22322 + 1163 WP_148713149.1 IS3-like element IS3 family transposase -
GC226_RS24735 (22353) 22353..22757 - 405 Protein_18 restriction endonuclease -
GC226_RS24740 (22935) 22935..23150 - 216 Protein_19 peptidase -
GC226_RS24745 (24241) 24241..24366 + 126 Protein_20 antirestriction protein -
GC226_RS24750 (24418) 24418..24609 + 192 WP_001027503.1 hypothetical protein -
GC226_RS24755 (25233) 25233..25517 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
GC226_RS24760 (25517) 25517..25807 + 291 WP_000421260.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GC226_RS24765 (25883) 25883..27115 + 1233 Protein_24 IS3 family transposase -
GC226_RS24770 (28038) 28038..28595 + 558 WP_000949484.1 type 1 fimbrial major subunit FimA -
GC226_RS24775 (28656) 28656..29204 + 549 WP_000277046.1 fimbrial protein -
GC226_RS24780 (29225) 29225..29980 + 756 WP_001369992.1 fimbria/pilus periplasmic chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..65782 65782
- inside IScluster/Tn - - 17923..27115 9192


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11356.12 Da        Isoelectric Point: 9.3504

>T141480 WP_000421260.1 NZ_CP045211:25517-25807 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELYGQTIY
VLRLWHTRENRYEPAP

Download         Length: 291 bp

>T141480 NZ_CP045211:25517-25807 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTTATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTATGAACCGGCCCCTTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT141480 WP_001178089.1 NZ_CP045211:25233-25517 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT141480 NZ_CP045211:25233-25517 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTTGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCTTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6SKD8

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References