Detailed information of TA system TA141479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 101062..101587
• Replicon: plasmid pO113-4
• Accession: NZ_CP045210
• Organism: Escherichia coli strain 4

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: GC226_RS24365
• Protein ID: WP_001159871.1
• Coordinates: 101062..101367 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q3ZU16
• Locus tag: GC226_RS24370
• Protein ID: WP_000813639.1
• Coordinates: 101369..101587 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GC226_RS24335 (GC226_24640)	97057..97230	+	174	Protein_104	DUF4113 domain-containing protein	-
GC226_RS24340 (GC226_24645)	97232..97648	-	417	WP_001278809.1	plasmid partitioning/stability family protein	-
GC226_RS24345 (GC226_24650)	97641..98621	-	981	WP_000688508.1	plasmid segregation protein ParM	-
GC226_RS24350 (GC226_24655)	99036..99344	-	309	WP_000030199.1	hypothetical protein	-
GC226_RS24355 (GC226_24660)	99431..100075	-	645	WP_001144036.1	ParA family protein	-
GC226_RS24360 (GC226_24665)	100255..101061	-	807	WP_000016966.1	site-specific integrase	-
GC226_RS24365 (GC226_24670)	101062..101367	-	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
GC226_RS24370 (GC226_24675)	101369..101587	-	219	WP_000813639.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
GC226_RS24375 (GC226_24680)	102183..102443	+	261	WP_000343083.1	hypothetical protein	-
GC226_RS24380 (GC226_24685)	102440..103030	+	591	WP_000194569.1	hypothetical protein	-
GC226_RS24385 (GC226_24690)	103048..103395	-	348	WP_000142426.1	DUF6404 family protein	-
GC226_RS24390 (GC226_24695)	103865..104185	-	321	WP_001365556.1	colicin-like pore-forming protein	-
GC226_RS24395 (GC226_24700)	104295..105026	-	732	WP_000850413.1	conjugal transfer complement resistance protein TraT	-
GC226_RS24400	105303..105455	-	153	Protein_117	IS481 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / espP	1..163406	163406

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11692.49 Da
• Isoelectric Point: 6.4674

>T141479 WP_001159871.1 NZ_CP045210:c101367-101062 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T141479 NZ_CP059963:229470-229745 [Pectobacterium brasiliense]
ATGGAAATACGTTGGACGCAAAAGGCACAGGATGACCTGGAACGTATTTATGGGTTTGCCAGCCAGTACAGTCGGCAACA
TGCTGACGAGGTGTTAGATCGTTTGATCATCGGCACCACGGGGCTTGCTGACTACCCGAGAATCGGCGTATCTCAGACAA
GGTATGAACCACGTGAGGTGAGAAAGATTTTGTTTGATGATTATGAAGTTCACTATGAAATTCAGCATGATACGAGTTAT
ATCGTCGATCTGTGGCATAAACGAGAAGACCGCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.30 Da
• Isoelectric Point: 4.5203

>AT141479 WP_000813639.1 NZ_CP045210:c101587-101369 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT141479 NZ_CP059963:229218-229466 [Pectobacterium brasiliense]
ATGGGACAACCCGCGAAACAGACGATCAGTGCTCAGATACCCGCCGAACTTGCGGCTGCGGTTGAAAGACTGGCTATTGA
ATTGGATAGATCCAAAAGCTGGGTGATTAAAGAAGCGCTGACCGCAATGATTGAAGAGAGAGAGCGGCGGCATCAGCGGA
TTTTGAAAGGTCTTGCTGATGTCGATGCGGGAAGAGTAGTAAGCCATGCAGATGTGATCGACTTCGCCAACAAACTGAAA
AAGTCATAA

Structures

Toxin

• AlphaFold DB: A0A829CCE8

Antitoxin

• AlphaFold DB: A0A7U9DIR5