Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4572754..4573554 Replicon chromosome
Accession NZ_CP045209
Organism Escherichia coli strain 4

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag GC226_RS22285 Protein ID WP_000342449.1
Coordinates 4572754..4573281 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag GC226_RS22290 Protein ID WP_001277108.1
Coordinates 4573288..4573554 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC226_RS22260 (4567829) 4567829..4568596 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
GC226_RS22265 (4568593) 4568593..4569870 - 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
GC226_RS22270 (4569867) 4569867..4570793 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
GC226_RS22275 (4570841) 4570841..4571950 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
GC226_RS22280 (4572374) 4572374..4572757 + 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
GC226_RS22285 (4572754) 4572754..4573281 - 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
GC226_RS22290 (4573288) 4573288..4573554 - 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
GC226_RS22295 (4573704) 4573704..4574807 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
GC226_RS22300 (4575078) 4575078..4575932 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
GC226_RS22305 (4576177) 4576177..4577235 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
GC226_RS22310 (4577228) 4577228..4577896 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T141478 WP_000342449.1 NZ_CP045209:c4573281-4572754 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T141478 NZ_CP059963:135228-135515 [Pectobacterium brasiliense]
ATGACCTATAAGTTAAAATTTGTGCCTTCTGCAATGAAGGAATGGAAAAAGCTAGGGCATCCTGTCCGGGAACAGTTCAA
GAAAAAGCTGGTTGAGCGTTTGGAAAATCCTCGTGTTCCTTCAGCCCAGCTTCATGGCCGTAAAGACCAGTATAAAATTA
AACTGAAGTCCGCTGGCTACAGGCTGGTGTATTTGGTCCAGGATGAGACCATTACTGTGATGGTTATGGGAGTTGGAAAG
CGCGAAGGTAGCCAGATTTATTCTGATACGAAAGGTCGCTCTGCTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT141478 WP_001277108.1 NZ_CP045209:c4573554-4573288 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT141478 NZ_CP059963:134984-135238 [Pectobacterium brasiliense]
ATGGCTTTCAACATCCTAACTAACACAGCCGCCAGTATTACTGAGTTGAAACGTGACCCGATGGGGACGGTTCGGGCTGC
TGAAGGTGAAACTATCGCTATACTGAACCGGAATGAGCCGGCGTTCTACTGTGTGCCTCCTGCTGTATTCGCCTATTTAA
TGGAACTGGCTGAAGATGCAGAACTGGGGCGTACCGTTGATGATCGGATGTCAGAGCTAGCTGACGCGGAAGAGATTTCG
CTCGATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References