Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3913769..3914423 Replicon chromosome
Accession NZ_CP045209
Organism Escherichia coli strain 4

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag GC226_RS19010 Protein ID WP_000244772.1
Coordinates 3913769..3914176 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GC226_RS19015 Protein ID WP_000354046.1
Coordinates 3914157..3914423 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC226_RS18990 (3909726) 3909726..3911459 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
GC226_RS18995 (3911465) 3911465..3912175 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GC226_RS19000 (3912200) 3912200..3913096 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GC226_RS19005 (3913208) 3913208..3913729 + 522 WP_001055874.1 flavodoxin FldB -
GC226_RS19010 (3913769) 3913769..3914176 - 408 WP_000244772.1 protein YgfX Toxin
GC226_RS19015 (3914157) 3914157..3914423 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GC226_RS19020 (3914666) 3914666..3915646 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
GC226_RS19025 (3915723) 3915723..3916382 - 660 WP_000250274.1 hemolysin III family protein -
GC226_RS19030 (3916546) 3916546..3916857 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
GC226_RS19035 (3916902) 3916902..3918335 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
GC226_RS19040 (3918392) 3918392..3919135 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T141475 WP_000244772.1 NZ_CP045209:c3914176-3913769 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T141475 NZ_CP059962:4959839-4960144 [Pectobacterium brasiliense]
ATGCCACAAGTGATTGTTTCAGAACGTGCAAGACGTGATTTGCAACGTCTGCAGGATTTTCTAAAAAACAAAAATGCGCT
GGCTGCGAAAAAGGCGGCTGAAGTTTTGATTCGGGGCATTCGTCAGTTAGAAGCGCTGCCGAATATCGGGCGACCCGTTG
AACATCTCCCTCTTGAATATCAGGAACTGGTGATCGAATTCGGCAGCAGCGGGTACGTGGTGCTTTACCGCCATGATGAA
CTGACTGACAGAGTGGTGATCCTGACCGTCAAGCATCAAAAAGAAGCCGGTTACCAAAACCCTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT141475 WP_000354046.1 NZ_CP045209:c3914423-3914157 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT141475 NZ_CP059962:4959561-4959851 [Pectobacterium brasiliense]
ATGGCTGACACGCCACGCCAAACCACGACGCTGCGCATCGATCAGGACATTAAAGAACGACTGAAAACGTTGGCCGATGA
CCGCCATAGCTCAGCCCATGCGCTAATGCTGGAAGCCATCACCGAATACGTGGAGCGTGAAGAAAAACGCAGTCAGTACC
GTAAAGAGGCGCTGGCCGCGTGGGAGGAATACCAGAGCACCGGCGAACACATCACCGCCGATGAGACGGTCACCTGGTTA
GAATCCTGGGGTTCTGGGCATGAACAGGATGCGCCTGCATGCCACAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References