Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3764347..3764930 Replicon chromosome
Accession NZ_CP045209
Organism Escherichia coli strain 4

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag GC226_RS18310 Protein ID WP_000254745.1
Coordinates 3764347..3764682 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag GC226_RS18315 Protein ID WP_000581937.1
Coordinates 3764682..3764930 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC226_RS18290 (3759816) 3759816..3760178 + 363 WP_000034929.1 hypothetical protein -
GC226_RS18295 (3760234) 3760234..3761532 - 1299 WP_000036723.1 phosphopyruvate hydratase -
GC226_RS18300 (3761620) 3761620..3763257 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
GC226_RS18305 (3763485) 3763485..3764276 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
GC226_RS18310 (3764347) 3764347..3764682 - 336 WP_000254745.1 endoribonuclease MazF Toxin
GC226_RS18315 (3764682) 3764682..3764930 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
GC226_RS18320 (3765008) 3765008..3767242 - 2235 WP_000226815.1 GTP pyrophosphokinase -
GC226_RS18325 (3767290) 3767290..3768591 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T141474 WP_000254745.1 NZ_CP045209:c3764682-3764347 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T141474 NZ_CP059962:4904844-4905119 [Pectobacterium brasiliense]
ATGGAAATACGTTGGACGCAAAAGGCACAGGATGACCTGGAACGTATTTATGGGTTTGCCAGCCAGTACAGTCGGCAACA
TGCTGACGAGGTGTTAGATCGTTTGATCATCGGCACCACGGGGCTTGCTGACTACCCGAGAATCGGCGTATCTCAGACAA
GGTATGAACCACGTGAGGTGAGAAAGATTTTGTTTGATGATTATGAAGTTCACTATGAAATTCAGCATGATACGAGTTAT
ATCGTCGATCTGTGGCATAAACGAGAAGACCGCTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT141474 WP_000581937.1 NZ_CP045209:c3764930-3764682 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT141474 NZ_CP059962:4904592-4904840 [Pectobacterium brasiliense]
ATGGGACAACCCGCGAAACAGACGATCAGTGCTCAGATACCCGCCGAACTTGCGGCTGCGGTTGAAAGACTGGCTATTGA
ATTGGATAGATCCAAAAGCTGGGTGATTAAAGAAGCGCTGACCGCAATGATTGAAGAGAGAGAGCGGCGGCATCAGCGGA
TTTTGAAAGGTCTTGCTGATGTCGATGCGGGAAGAGTAGTAAGCCATGCAGATGTGATCGACTTCGCCAACAAACTGAAA
AAGTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References