Detailed information of TA system TA141473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3465913..3466538
• Replicon: chromosome
• Accession: NZ_CP045209
• Organism: Escherichia coli strain 4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GC226_RS16855
• Protein ID: WP_000911330.1
• Coordinates: 3465913..3466311 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: GC226_RS16860
• Protein ID: WP_000450524.1
• Coordinates: 3466311..3466538 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GC226_RS16835 (3461791)	3461791..3461991	+	201	WP_000383836.1	YpfN family protein	-
GC226_RS16840 (3462101)	3462101..3462799	-	699	WP_000679807.1	esterase	-
GC226_RS16845 (3462873)	3462873..3464888	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
GC226_RS16850 (3464903)	3464903..3465766	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
GC226_RS16855 (3465913)	3465913..3466311	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
GC226_RS16860 (3466311)	3466311..3466538	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
GC226_RS16865 (3466692)	3466692..3467405	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
GC226_RS16870 (3467618)	3467618..3468652	-	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
GC226_RS16875 (3468669)	3468669..3469547	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
GC226_RS16880 (3469693)	3469693..3470265	+	573	WP_000176191.1	glycine cleavage system transcriptional repressor	-
GC226_RS16885 (3470265)	3470265..3470735	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T141473 WP_000911330.1 NZ_CP045209:c3466311-3465913 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T141473 NZ_CP059962:4810602-4810889 [Pectobacterium brasiliense]
ATGACCTATAAGTTAAAATTTGTGCCTTCTGCAATGAAGGAATGGAAAAAGCTAGGGCATCCTGTCCGGGAACAGTTCAA
GAAAAAGCTGGTTGAGCGTTTGGAAAATCCTCGTGTTCCTTCAGCCCAGCTTCATGGCCGTAAAGACCAGTATAAAATTA
AACTGAAGTCCGCTGGCTACAGGCTGGTGTATTTGGTCCAGGATGAGACCATTACTGTGATGGTTATGGGAGTTGGAAAG
CGCGAAGGTAGCCAGATTTATTCTGATACGAAAGGTCGCTCTGCTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT141473 WP_000450524.1 NZ_CP045209:c3466538-3466311 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT141473 NZ_CP059962:4810358-4810612 [Pectobacterium brasiliense]
ATGGCTTTCAACATCCTAACTAACACAGCCGCCAGTATTACTGAGTTGAAACGTGACCCGATGGGGACGGTTCGGGCTGC
TGAAGGTGAAACTATCGCTATACTGAACCGGAATGAGCCGGCGTTCTACTGTGTGCCTCCTGCTGTATTCGCCTATTTAA
TGGAACTGGCTGAAGATGCAGAACTGGGGCGTACCGTTGATGATCGGATGTCAGAGCTAGCTGACGCGGAAGAGATTTCG
CTCGATGACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33