Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1230250..1230868 Replicon chromosome
Accession NZ_CP045209
Organism Escherichia coli strain 4

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GC226_RS05780 Protein ID WP_001291435.1
Coordinates 1230250..1230468 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GC226_RS05785 Protein ID WP_000344800.1
Coordinates 1230494..1230868 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC226_RS05745 (1225539) 1225539..1226111 + 573 WP_000779831.1 YbaY family lipoprotein -
GC226_RS05750 (1226142) 1226142..1226453 - 312 WP_000409911.1 MGMT family protein -
GC226_RS05760 (1226832) 1226832..1227185 + 354 WP_000878140.1 DUF1428 domain-containing protein -
GC226_RS05765 (1227227) 1227227..1228777 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GC226_RS05770 (1228941) 1228941..1229411 - 471 WP_000136192.1 YlaC family protein -
GC226_RS05775 (1229527) 1229527..1230078 - 552 WP_000102568.1 maltose O-acetyltransferase -
GC226_RS05780 (1230250) 1230250..1230468 - 219 WP_001291435.1 HHA domain-containing protein Toxin
GC226_RS05785 (1230494) 1230494..1230868 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GC226_RS05790 (1231414) 1231414..1234563 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
GC226_RS05795 (1234586) 1234586..1235779 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T141461 WP_001291435.1 NZ_CP045209:c1230468-1230250 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T141461 NZ_CP059962:164118-164531 [Pectobacterium brasiliense]
GTGGCCCAGTGGCGATGTGACCTCCGCGTTTCCTGGCGCATGCAGCTGTTATCATTGGTTGTGCATGGCCTATTAGTATT
GCTCATTCTGCTGGCACCGTGGCCGGACGGCTATGCATGGCTATGGCTATGCCTGGTCACGATGGTGATGTTCGGTTTTA
TCCGTAGCCAACGGAATATTAAATCCCGACAGGGGGAAATCGTTCTGCTCAGCGAAACGACCCTGAACTGGCGGCAGCAG
GAATGGCAGATCGTTAAACGACCGTGGCTGCTGAAGAATGGCATATTACTGTCATTGCAAGCGGTTAACGGTAAAGACAA
GCAACAGCTGTGGCTGGCATCGGACAGCATGGGTGATGACGAATGGCGTCACTTGCGTCAAATTCTTTTGCAGCAGAAAC
ACTGGGCAAGATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT141461 WP_000344800.1 NZ_CP045209:c1230868-1230494 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT141461 NZ_CP059962:163871-164137 [Pectobacterium brasiliense]
ATGGAAATCGATAATAAATCACGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAATTGGATATCGCAATCATGCCGTT
TTTTGAGCATGACTATGACACACTTAATGACACTGATAAGCGCACGTTTGTACGCTTGTTACAAAGCGACGATCCCGATT
TATTCAACTGGCTGATGAACCACGGTGAACCGGAGGACGGAGAGCTGAAACGCATGATTTCCCTTATTCAGACGCGAAAT
AAAGATCGTGGCCCAGTGGCGATGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References