Detailed information of TA system TA141460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1195942..1196779
• Replicon: chromosome
• Accession: NZ_CP045209
• Organism: Escherichia coli strain 4

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: GC226_RS05610
• Protein ID: WP_000227784.1
• Coordinates: 1195942..1196484 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: GC226_RS05615
• Protein ID: WP_001297137.1
• Coordinates: 1196468..1196779 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GC226_RS05590 (1191481)	1191481..1192392	-	912	WP_000705853.1	2-dehydropantoate 2-reductase	-
GC226_RS05595 (1192560)	1192560..1193051	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
GC226_RS05600 (1193179)	1193179..1194543	-	1365	WP_001000978.1	MFS transporter	-
GC226_RS05605 (1194951)	1194951..1195886	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
GC226_RS05610 (1195942)	1195942..1196484	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
GC226_RS05615 (1196468)	1196468..1196779	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
GC226_RS05620 (1196964)	1196964..1197854	-	891	WP_000971336.1	heme o synthase	-
GC226_RS05625 (1197866)	1197866..1198195	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
GC226_RS05630 (1198195)	1198195..1198809	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
GC226_RS05635 (1198799)	1198799..1200790	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
GC226_RS05640 (1200812)	1200812..1201759	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T141460 WP_000227784.1 NZ_CP045209:c1196484-1195942 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T141460 NZ_CP059961:4716881-4717225 [Pectobacterium brasiliense]
ATGAAATCACTCTACTGGGTTGGCAGTAGCAAAAAGGATCTCCAATCGCTACCTGCAGATGTGCAGGATGTGTTCGGTTA
CGCACTTCATGTGGCTCAGACTGGTGGTAAGCACACTCAAGCCAAGCCGCTTAAAGGTTTTGGTGGTGCAGGCGTATTAG
AAGTCGTTGAGGATTATATCGGCGATACTTATCGCGCAGTCTACACCGTCAAATTTGGAGAGGCAGTGTACGTTCTACAT
ACTTTTCAGAAAAAATCGTCATCTGGAATTGCAACACCGAAATCCGATATGAACACCATCCGTGAGCGCCTGAAAACCGC
AGAAAACCATGCAAAAGGAGCATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT141460 WP_001297137.1 NZ_CP045209:c1196779-1196468 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT141460 NZ_CP059961:4717227-4717544 [Pectobacterium brasiliense]
ATGAGCCTGGATATTGAAGTCAGCAGCGGTAATGTGTACGCCGACCTTGGCAACGTGCATGCTGAAGATATGTTGGTCAA
AGCGCAGCTTGCCACACTGATTAGCTCAATCATCAAAAGCCGCCGTCTCACACAGGCAGAAGCAGCGAAGCTATTGGGCA
TGCCCCAACCCAAACTATCCAACTTATTACGTGGTCAGTTCAGGGGAATCAGTGAAACCAAAATGTTGGAATGCCTCACG
AAGCTGGGACGCGATATACAGATCGTGGTAGGGAAGCCGCGCCGCACGCCGGGTAGCGTTAAAGTCGTGTTTGCGTAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3