Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 461877..462374 Replicon chromosome
Accession NZ_CP045209
Organism Escherichia coli strain 4

Toxin (Protein)


Gene name ghoT Uniprot ID -
Locus tag GC226_RS02180 Protein ID WP_001173344.1
Coordinates 462201..462374 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag GC226_RS02175 Protein ID WP_000398619.1
Coordinates 461877..462173 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GC226_RS02155 (458608) 458608..459327 - 720 WP_000611288.1 two-component system response regulator DcuR -
GC226_RS02160 (459324) 459324..460955 - 1632 WP_001216461.1 sensor histidine kinase -
GC226_RS02165 (461136) 461136..461366 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
GC226_RS02170 (461378) 461378..461650 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
GC226_RS02175 (461877) 461877..462173 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GC226_RS02180 (462201) 462201..462374 + 174 WP_001173344.1 type V toxin-antitoxin system toxin GhoT Toxin
GC226_RS02185 (462493) 462493..464010 - 1518 WP_001295074.1 lysine--tRNA ligase -
GC226_RS02190 (464247) 464247..465704 - 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6583.12 Da        Isoelectric Point: 10.2288

>T141457 WP_001173344.1 NZ_CP045209:462201-462374 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSVFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T141457 NZ_CP059961:2333379-2333954 [Pectobacterium brasiliense]
ATGAATCACTCCCCCTATCCTGTGGTACTTGATGCGTGTGTCATTTATCCATCTTTGCTACGGGATTTGCTCATGCATCT
TGGTTTAACAGGGCTATATCAGCCAAAGTGGACGGTACTAATTCAGGAAGAGTGGCAGCGAAATTTGTTGCTAAATAATG
CAGACGTAACGATGGATAAGCTCAGGAACATTGAGCATTTGATGAATAAGGCATTACCTGATGCCAATGTGACTGGGTTC
GAACAACTTATCCCTGCTCTGGTTTTACCTGATGCGGATGATCGACATGTATTGGCCGCTGCGATACGCGCTAATGCAGA
AGTTATCGTCACGGCAAATCTGAAAGATTTTCCAGCCACGTCACTTTCCGAATTTGGAGTGGAAGCCATGCACCCAGACG
AATTCATTGCCGATTTATTTGATCTCAATTCTGCACTGGTGCTTAAAGCCGTTCGAACTTCGCGAACAGCCTTCAGAAAG
CCTCCATTTGATGTGGACGAGTATCTGGATTGTCTCCTGCGTCAGGGGTTACCTATGACGGTAAAAGAACTGGATGCGTA
TCGAGCACTGATTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT141457 WP_000398619.1 NZ_CP045209:461877-462173 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT141457 NZ_CP059961:2332918-2333379 [Pectobacterium brasiliense]
ATGAAAAATACTCCTCTAAATAGTTTGAGCCTTCCGGCTCCAAAAGAAATGGAGGCCGCTATCCGTGGACAAAGGCAGCT
TGCAACTTACCTGTCTACGAAGCTGGAAACACAGGAAATTTCGATTCAGGATGCGCACGACATTGCCCATCGGATCGAGT
TGCCTACCTCAGCACTCACGCTTTTGATGAGTATCCTTGGCGAACTGGCGGCGGGTAATGCTGTCCAGATTGTCCCTGTG
CAGGCTGAATTAACGACACAAGAAGCGGCAAATATTCTGAATGTTTCACGGCCACATATGGTGAAGCTTTTGGAAGAAGG
CCATCTTCCTTTCCATAAAACAGGTCGTCATCGCCGCGTGCTTTTTGCTGATTTGATGCAATACAAAGAACAGCGTGAAA
ATGAAAGTATGCAAGCCATGCAGGAGCTGACAGACCGAAGTCAGGAATTCGGGTTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References