Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 171808..172451 Replicon plasmid unnamed1
Accession NZ_CP045207
Organism Escherichia coli strain DE17

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag GBG22_RS24920 Protein ID WP_001034044.1
Coordinates 172035..172451 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag GBG22_RS24915 Protein ID WP_001261286.1
Coordinates 171808..172038 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GBG22_RS24900 167013..167243 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
GBG22_RS24905 167240..167656 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
GBG22_RS24910 167701..171495 - 3795 WP_001144732.1 hypothetical protein -
GBG22_RS24915 171808..172038 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GBG22_RS24920 172035..172451 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
GBG22_RS24925 172526..174091 + 1566 WP_001128474.1 AAA family ATPase -
GBG22_RS24930 174076..175098 + 1023 WP_000361402.1 helicase UvrD -
GBG22_RS24935 175352..176049 - 698 Protein_186 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..194162 194162
- flank IS/Tn - - 175352..175855 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T141455 WP_001034044.1 NZ_CP045207:172035-172451 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T141455 NZ_CP059961:c1603849-1603544 [Pectobacterium brasiliense]
ATGCAATTCATTGTTTATCAATACAAACGCGCCAGTCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ACCAAAGCGGCGTATGGTCATCCCGCTTATCGAAGCATATCACCTGTCTGAGAAAGTGAATAAAACATTGTTCCCTTTGA
TCCGCATCGACGGCGAAGATTATCGGCTGATGACAACTGAACTATCGAGCGTTCCCGTTGAGGTTATTGGTGAAGTGACT
GCGGATCTTGGCGACTACGCGGATGAGATTAAGGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT141455 WP_001261286.1 NZ_CP045207:171808-172038 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT141455 NZ_CP059961:c1604070-1603852 [Pectobacterium brasiliense]
ATGAAACACCGCGTTAGTGTTACCGTAGACAAAGACAATTATCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCCGGGCT
GGTGAATGACGCCATTGGCAAAGAAGCCCGCCGTATCAAGGCTGAGGAGTGGAAGAAGGAAAACCGCGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCGCAGAATGGCTCCTTTGCAGATGAAAACAGGAATTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References