Detailed information of TA system TA141454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 167013..167656
• Replicon: plasmid unnamed1
• Accession: NZ_CP045207
• Organism: Escherichia coli strain DE17

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: C7S9Y5
• Locus tag: GBG22_RS24905
• Protein ID: WP_001034046.1
• Coordinates: 167240..167656 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: V0SR71
• Locus tag: GBG22_RS24900
• Protein ID: WP_001261278.1
• Coordinates: 167013..167243 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBG22_RS24870	162804..163151	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
GBG22_RS24875	163151..163828	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
GBG22_RS24880	163883..164128	+	246	Protein_175	hypothetical protein	-
GBG22_RS24885	164162..165295	-	1134	WP_000545987.1	DUF3800 domain-containing protein	-
GBG22_RS24890	165462..166235	-	774	WP_000905949.1	hypothetical protein	-
GBG22_RS24895	166248..166748	-	501	WP_000528932.1	hypothetical protein	-
GBG22_RS24900	167013..167243	+	231	WP_001261278.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GBG22_RS24905	167240..167656	+	417	WP_001034046.1	type II toxin-antitoxin system VapC family toxin	Toxin
GBG22_RS24910	167701..171495	-	3795	WP_001144732.1	hypothetical protein	-
GBG22_RS24915	171808..172038	+	231	WP_001261286.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
GBG22_RS24920	172035..172451	+	417	WP_001034044.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..194162	194162

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14978.31 Da
• Isoelectric Point: 6.7113

>T141454 WP_001034046.1 NZ_CP045207:167240-167656 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Nucleotide

• Length: 417 bp

>T141454 NZ_CP059961:1599775-1600269 [Pectobacterium brasiliense]
ATGATCTCCGCTCCTGAGCCACTCCGCGCCGAACATATACTATCCTCATTCTGTTGCGGCGTGGAGTCTATGGACAACTG
GCTGAAACAACGGGCGATGAAAAATCAGGTCACTGGCGCATCCCGAACTTTCGTCAGTTGCGATGATTCAAAAGTGTTGG
CGTATTACTCATTGGCTTCCAGCGCCGTGGCAACGAATGCCGCCCCCGGACGCTTTCGTCGCAATATGCCCGATCCGATC
CCGGTGGTAGTGCTGGGACGTCTGGCGGTTGATACGTCATTGCATGGTCAAGGTGTTGGACGGGCATTGGTAAGAGATGC
TGGGCTACGGGTGATTCAGGTTGCGGATACTATCGGTATTCGCGGGATGTTAGTTCATGCGCTGTCTGACGAAGCGCGGG
AGTTTTACCTACGAGTGGGGTTTGAGCCTTCGCCGATTGACCCGATGATGTTGATGGTGACGTTAGGGGATTTGGTGGGG
AGTTTATCAATTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8853.07 Da
• Isoelectric Point: 4.6437

>AT141454 WP_001261278.1 NZ_CP045207:167013-167243 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Nucleotide

• Length: 231 bp

>AT141454 NZ_CP059961:1599485-1599778 [Pectobacterium brasiliense]
ATGCCAGCGACGAATAGCGTTTCTGTTAAACGTGAAACATTGAATTTACGCATCAAGCCCGCCGAACGCGACCTTATCGA
CCGTGCGGCGAAGGCCAGAGGAAAAAATCGCACTGATTTTGTCCTCGAAGCTGCTCGTGCCGCTGCAGAGGAAGCGTTGA
TCGATCAGCGCATCATCATGGCAGATCCCGACGCTTATCAGGAATTCCTTGCCCGCCTGGATCAGGCTCCTGCACCAAAT
GCCGCGCTGCGAAAAACCATGCAAACGCCTGCACCGTGGGAACAGAAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A7U9NXF9

Antitoxin

• AlphaFold DB: V0SR71