Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 159818..160343 Replicon plasmid unnamed1
Accession NZ_CP045207
Organism Escherichia coli strain DE17

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag GBG22_RS24850 Protein ID WP_001159871.1
Coordinates 159818..160123 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag GBG22_RS24855 Protein ID WP_000813630.1
Coordinates 160125..160343 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GBG22_RS24835 155773..156978 - 1206 WP_001442122.1 AAA family ATPase -
GBG22_RS24840 157600..158331 + 732 WP_000504262.1 replication initiation protein -
GBG22_RS24845 159011..159817 - 807 WP_000016968.1 site-specific integrase -
GBG22_RS24850 159818..160123 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
GBG22_RS24855 160125..160343 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
GBG22_RS24860 160897..161154 - 258 WP_040111990.1 hypothetical protein -
GBG22_RS24865 161213..162784 - 1572 WP_000381395.1 IS66 family transposase -
GBG22_RS24870 162804..163151 - 348 WP_000624622.1 IS66 family insertion sequence element accessory protein TnpB -
GBG22_RS24875 163151..163828 - 678 WP_001339397.1 IS66 family insertion sequence hypothetical protein -
GBG22_RS24880 163883..164128 + 246 Protein_175 hypothetical protein -
GBG22_RS24885 164162..165295 - 1134 WP_000545987.1 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..194162 194162


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T141453 WP_001159871.1 NZ_CP045207:c160123-159818 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T141453 NZ_CP059961:1529299-1529604 [Pectobacterium brasiliense]
ATGCCACAAGTGATTGTTTCAGAACGTGCAAGACGTGATTTGCAACGTCTGCAGGATTTTCTAAAAAACAAAAATGCGCT
GGCTGCGAAAAAGGCGGCTGAAGTTTTGATTCGGGGCATTCGTCAGTTAGAAGCGCTGCCGAATATCGGGCGACCCGTTG
AACATCTCCCTCTTGAATATCAGGAACTGGTGATCGAATTCGGCAGCAGCGGGTACGTGGTGCTTTACCGCCATGATGAA
CTGACTGACAGAGTGGTGATCCTGACCGTCAAGCATCAAAAAGAAGCCGGTTACCAAAACCCTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT141453 WP_000813630.1 NZ_CP045207:c160343-160125 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT141453 NZ_CP059961:1529021-1529311 [Pectobacterium brasiliense]
ATGGCTGACACGCCACGCCAAACCACGACGCTGCGCATCGATCAGGACATTAAAGAACGACTGAAAACGTTGGCCGATGA
CCGCCATAGCTCAGCCCATGCGCTAATGCTGGAAGCCATCACCGAATACGTGGAGCGTGAAGAAAAACGCAGTCAGTACC
GTAAAGAGGCGCTGGCCGCGTGGGAGGAATACCAGAGCACCGGCGAACACATCACCGCCGATGAGACGGTCACCTGGTTA
GAATCCTGGGGTTCTGGGCATGAACAGGATGCGCCTGCATGCCACAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References