Detailed information of TA system TA141448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 110502..110878
• Replicon: plasmid unnamed1
• Accession: NZ_CP045207
• Organism: Escherichia coli strain DE17

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: GBG22_RS24570
• Protein ID: WP_000969988.1
• Coordinates: 110696..110878 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: GBG22_RS24565
• Protein ID: WP_001324224.1
• Coordinates: 110502..110699 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBG22_RS24545	105762..105998	+	237	WP_001323890.1	colicin V immunity protein	-
GBG22_RS24550	105976..106287	+	312	WP_001259758.1	colicin V	-
GBG22_RS24555	106457..108571	-	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
GBG22_RS24560	108546..109820	-	1275	WP_000489608.1	HlyD family secretion protein	-
GBG22_RS24565	110502..110699	+	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
GBG22_RS24570	110696..110878	+	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GBG22_RS24575	110977..111285	+	309	WP_000377483.1	hypothetical protein	-
GBG22_RS24580	111845..112879	+	1035	WP_001190233.1	3-deoxy-7-phosphoheptulonate synthase	-
GBG22_RS24585	113604..114281	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
GBG22_RS24590	114281..114628	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..194162	194162

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-113)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-87)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T141448 WP_000969988.1 NZ_CP045207:110696-110878 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T141448 NZ_CP059961:892664-893050 [Pectobacterium brasiliense]
ATGGAGGTTTACAGATTGGCATGGGAAATAGTCACACGGGCACTATTCGATACGTGGTTTGATGAGCAAACTGAAACGGT
ACAGGAAGAGGTTTTGGCACACCTCGCTATACTCGAAGAGGATGGCCCACAGCTAGGGCGACCACAGGTTGATCAGATTA
AAGGCTCAAAATACAAAAATATGAAGGAGCTTCGAGTTCAGGTTGGCGGCCACCCATTCCGAACATTCTTCGCGTTTGAC
CGGACACGTAAAGCGATTGTTTTATGTGCGGGAGATAAGAAAGGCGAAAATGCAAAGTTGTTCTACGAACGTATGATCAA
GATCGCAGACGCTGAGTTTGCATCGCATCTGATATCACCGGAGGTAAAAGAAGATGGCGACGCTTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT141448 WP_001324224.1 NZ_CP045207:110502-110699 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT141448 NZ_CP059961:893037-893336 [Pectobacterium brasiliense]
ATGGCGACGCTTAAAGAAATGATGGCTAAACGTTCACCGGAGAGCCGCGCCCGCATTGAAGCGCGCACGGCTGAGATCCG
TCAGGAAATCACTTTAGCGAAACTACGCGAAGAGCTGAACATGTCGCAGACTCAGCTTGCGTTAGCATTGGGCGTCAAGC
AGCCATCTATTGTTAAGATGGAGAAGGTCGAAAATGACCCAAAACTGTCAACGCTGAAACGCTATGTCACTGCCCTAGGC
GGCAAGCTTACGCTAGATGTGACCTTGCCTGATGGTAAGCGTATCGCGCTTACTTTGTAA

Structures

Toxin

• AlphaFold DB: A0A075MDK3