Detailed information of TA system TA141446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 38225..38850
• Replicon: plasmid unnamed1
• Accession: NZ_CP045207
• Organism: Escherichia coli strain DE17

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GBG22_RS24160
• Protein ID: WP_000911333.1
• Coordinates: 38225..38623 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: GBG22_RS24165
• Protein ID: WP_000450520.1
• Coordinates: 38623..38850 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBG22_RS24145	35401..36972	-	1572	WP_000381395.1	IS66 family transposase	-
GBG22_RS24150	36992..37339	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
GBG22_RS24155	37339..38016	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
GBG22_RS24160	38225..38623	-	399	WP_000911333.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GBG22_RS24165	38623..38850	-	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..194162	194162

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14919.19 Da
• Isoelectric Point: 7.8605

>T141446 WP_000911333.1 NZ_CP045207:c38623-38225 [Escherichia coli]
MLKFMLDTNICIFTMKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T141446 NZ_CP059961:c758972-758697 [Pectobacterium brasiliense]
ATGCAGGGAAAAATAGCCAGCCTACGAAAGAAGCAGCGGGAAACGCTGATCCAAATTTTCAAAACACCAGTACTTTCAGG
TGTTAAATGGTCGAATATTGAGTCACTCGTTACTGCGTTAGGCGGTGAAATTAAGGAAGGTAGCGGATCGCGAGTGCGAT
TTTTATTGAACGGGAGTATCGCCCGGTTCCATCGTCCCCATCCGTCACCGGATACTGATAAAGGTGCACTGGTTAGCCTG
CGTGAATGGCTGGAAAGTATAGGAGTAAAACCATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT141446 WP_000450520.1 NZ_CP045207:c38850-38623 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT141446 NZ_CP059961:c758700-758338 [Pectobacterium brasiliense]
ATGACGAAAGCACTCAACACACCAAATACGATGGTTGTTTCTGGACAACCGGCCATTATCAGTTATGTACCGGAAATGGG
TATGTTTCGCGGTAAGTTTATTGGTTTATCTGGCTACTGTGATTTTGTCGCCGACAGTATTAATGGCTTGAGAAGCGAGG
GTGAAATTTCGCTACGCGAATATCTGGAAGACTGTCAGGAAAATGGCATTGAACCTTACGAGCGTGAAGAGAAGGTGAAA
ACATTCACCCTCCGCTACCCTGAATCATTTGGGGAACGACTGACTATTGCGGCTGCTGAGCGCCAAATTTCAGTGAATAC
GTTTATTGTTGAAACGCTTAGCGAACACCTGAAACAGGCTTAA

Structures

Antitoxin

• AlphaFold DB: V0VCB2