Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4422513..4423010 Replicon chromosome
Accession NZ_CP045206
Organism Escherichia coli strain DE17

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag GBG22_RS21610 Protein ID WP_001173343.1
Coordinates 4422513..4422686 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag GBG22_RS21615 Protein ID WP_001312331.1
Coordinates 4422714..4423010 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GBG22_RS21600 4419183..4420640 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
GBG22_RS21605 4420877..4422394 + 1518 WP_000003806.1 lysine--tRNA ligase -
GBG22_RS21610 4422513..4422686 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
GBG22_RS21615 4422714..4423010 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GBG22_RS21620 4423239..4423511 - 273 WP_000405647.1 N-acetyltransferase -
GBG22_RS21625 4423523..4423753 - 231 WP_000373293.1 (4Fe-4S)-binding protein -
GBG22_RS21630 4423934..4425565 + 1632 WP_001216482.1 sensor histidine kinase -
GBG22_RS21635 4425562..4426281 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4368413..4423010 54597


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T141444 WP_001173343.1 NZ_CP045206:c4422686-4422513 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T141444 NZ_CP059961:c87590-87174 [Pectobacterium brasiliense]
GTGAGTCGCTTGTTCATGCTGGATACCAATATCTGTTCCTTCATTATGCGGGAACACCCTGCGGAGGTGATTGAAAAGTT
GCAGCAGTGCACGCTGCGGCGTGATCGGATTGTGGTCTCTGCCATCACTTACGCTGAAATGCGTTTCGGGGCGGTTGGTA
AAAAAGCCTCGCCACGCCATATGCAACTGGTCGATGCTTTTTGCCGTCGGCTCGATGCGGTATTACCTTGGGATAGCGCC
GCAGTAGATGCCACCACGAAAATCAAAATCGAGCTAGCCGCCGCTGGCACACCTATTGGTCCAAACGATACGGCCATCGC
GGGCCATGCCATCAGCGCTAAGGCCATATTAGTCACCAATAATCTGAGAGAATTTGAGCGAGTACCGGGGCTGACGTTGG
AAGATTGGGTTAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT141444 WP_001312331.1 NZ_CP045206:c4423010-4422714 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT141444 NZ_CP059961:c87814-87587 [Pectobacterium brasiliense]
ATGCGTATTGTTTCGGTATTTAAAAACGGTAACAACCGTGCCATTCGCTTGCCGCGTGATATGGACTTTGAAGGGGTCAA
CGAACTGGAGATTGTGCGTGAGGGAGATACCATCATGCTGCGTCCTGTTAAGCCCAATTGGCATTCGTTTTTGCAAGAAG
ATCGCGCTGACGCCGACTTTATGACAGAACGTCAGGATGTGGTCGAGGAGGGGAGATTCAAACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References