Detailed information of TA system TA141440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3979869..3980227
• Replicon: chromosome
• Accession: NZ_CP045206
• Organism: Escherichia coli strain DE17

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: GBG22_RS19495
• Protein ID: WP_001332330.1
• Coordinates: 3979869..3979991 (-)
• Length: 41 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: GBG22_RS19500
• Protein ID: WP_000125566.1
• Coordinates: 3979994..3980227 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBG22_RS19465	3975351..3976637	+	1287	WP_039267775.1	peptidylprolyl isomerase SurA	-
GBG22_RS19470	3976637..3977626	+	990	WP_000241229.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
GBG22_RS19475	3977623..3978444	+	822	WP_001065363.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
GBG22_RS19480	3978447..3978824	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
GBG22_RS19485	3978831..3979679	+	849	WP_000257201.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
GBG22_RS19490	3979676..3979828	-	153	WP_146692624.1	CcdB family protein	-
GBG22_RS19495	3979869..3979991	-	123	WP_001332330.1	CcdB family protein	Toxin
GBG22_RS19500	3979994..3980227	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
GBG22_RS19505	3980313..3980792	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
GBG22_RS19510	3980984..3982846	-	1863	WP_000377122.1	glutathione-regulated potassium-efflux system protein KefC	-
GBG22_RS19515	3982839..3983369	-	531	WP_000600749.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
GBG22_RS19520	3983477..3984808	-	1332	WP_001183207.1	MFS transporter	-
GBG22_RS19525	3984867..3985154	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-39)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4563.34 Da
• Isoelectric Point: 10.9266

>T141440 WP_001332330.1 NZ_CP045206:c3979991-3979869 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPI

Nucleotide

• Length: 123 bp

>T141440 NZ_CP059960:2557783-2558358 [Pectobacterium brasiliense]
ATGAATCACTCCCCCTATCCTGTGGTACTTGATGCGTGTGTCATTTATCCATCTTTGCTACGGGATTTGCTCATGCATCT
TGGTTTAACAGGGCTATATCAGCCAAAGTGGACGGTACTAATTCAGGAAGAGTGGCAGCGAAATTTGTTGCTAAATAATG
CAGACGTAACGATGGATAAGCTCAGGAACATTGAGCATTTGATGAATAAGGCATTACCTGATGCCAATGTGACTGGGTTC
GAACAACTTATCCCTGCTCTGGTTTTACCTGATGCGGATGATCGACATGTATTGGCCGCTGCGATACGCGCTAATGCAGA
AGTTATCGTCACGGCAAATCTGAAAGATTTTCCAGCCACGTCACTTTCCGAATTTGGAGTGGAAGCCATGCACCCAGACG
AATTCATTGCCGATTTATTTGATCTCAATTCTGCACTGGTGCTTAAAGCCGTTCGAACTTCGCGAACAGCCTTCAGAAAG
CCTCCATTTGATGTGGACGAGTATCTGGATTGTCTCCTGCGTCAGGGGTTACCTATGACGGTAAAAGAACTGGATGCGTA
TCGAGCACTGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT141440 WP_000125566.1 NZ_CP045206:c3980227-3979994 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT141440 NZ_CP059960:2557322-2557783 [Pectobacterium brasiliense]
ATGAAAAATACTCCTCTAAATAGTTTGAGCCTTCCGGCTCCAAAAGAAATGGAGGCCGCTATCCGTGGACAAAGGCAGCT
TGCAACTTACCTGTCTACGAAGCTGGAAACACAGGAAATTTCGATTCAGGATGCGCACGACATTGCCCATCGGATCGAGT
TGCCTACCTCAGCACTCACGCTTTTGATGAGTATCCTTGGCGAACTGGCGGCGGGTAATGCTGTCCAGATTGTCCCTGTG
CAGGCTGAATTAACGACACAAGAAGCGGCAAATATTCTGAATGTTTCACGGCCACATATGGTGAAGCTTTTGGAAGAAGG
CCATCTTCCTTTCCATAAAACAGGTCGTCATCGCCGCGTGCTTTTTGCTGATTTGATGCAATACAAAGAACAGCGTGAAA
ATGAAAGTATGCAAGCCATGCAGGAGCTGACAGACCGAAGTCAGGAATTCGGGTTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9