Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3531425..3532043 Replicon chromosome
Accession NZ_CP045206
Organism Escherichia coli strain DE17

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GBG22_RS17350 Protein ID WP_001291435.1
Coordinates 3531825..3532043 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GBG22_RS17345 Protein ID WP_000344800.1
Coordinates 3531425..3531799 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GBG22_RS17335 3526515..3527708 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GBG22_RS17340 3527731..3530880 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
GBG22_RS17345 3531425..3531799 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GBG22_RS17350 3531825..3532043 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GBG22_RS17355 3532217..3532768 + 552 WP_000102543.1 maltose O-acetyltransferase -
GBG22_RS17360 3532884..3533354 + 471 WP_000136192.1 YlaC family protein -
GBG22_RS17365 3533518..3535068 + 1551 WP_001350616.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GBG22_RS17370 3535110..3535463 - 354 WP_000878140.1 DUF1428 domain-containing protein -
GBG22_RS17380 3535842..3536153 + 312 WP_000409908.1 MGMT family protein -
GBG22_RS17385 3536184..3536756 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T141438 WP_001291435.1 NZ_CP045206:3531825-3532043 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T141438 NZ_CP059960:c1938927-1938622 [Pectobacterium brasiliense]
ATGCAATTCATTGTTTATCAATACAAACGCGCCAGTCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ACCAAAGCGGCGTATGGTCATCCCGCTTATCGAAGCATATCACCTGTCTGAGAAAGTGAATAAAACATTGTTCCCTTTGA
TCCGCATCGACGGCGAAGATTATCGGCTGATGACAACTGAACTATCGAGCGTTCCCGTTGAGGTTATTGGTGAAGTGACT
GCGGATCTTGGCGACTACGCGGATGAGATTAAGGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT141438 WP_000344800.1 NZ_CP045206:3531425-3531799 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT141438 NZ_CP059960:c1939148-1938930 [Pectobacterium brasiliense]
ATGAAACACCGCGTTAGTGTTACCGTAGACAAAGACAATTATCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCCGGGCT
GGTGAATGACGCCATTGGCAAAGAAGCCCGCCGTATCAAGGCTGAGGAGTGGAAGAAGGAAAACCGCGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCGCAGAATGGCTCCTTTGCAGATGAAAACAGGAATTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References