Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1047988..1048571 Replicon chromosome
Accession NZ_CP045206
Organism Escherichia coli strain DE17

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag GBG22_RS04960 Protein ID WP_000254750.1
Coordinates 1048236..1048571 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag GBG22_RS04955 Protein ID WP_000581937.1
Coordinates 1047988..1048236 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GBG22_RS04945 1044327..1045628 + 1302 Protein_969 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
GBG22_RS04950 1045676..1047910 + 2235 WP_000226815.1 GTP pyrophosphokinase -
GBG22_RS04955 1047988..1048236 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
GBG22_RS04960 1048236..1048571 + 336 WP_000254750.1 endoribonuclease MazF Toxin
GBG22_RS04965 1048643..1049434 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
GBG22_RS04970 1049662..1051299 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
GBG22_RS04975 1051387..1052685 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T141420 WP_000254750.1 NZ_CP045206:1048236-1048571 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T141420 NZ_CP059959:1221463-1221750 [Pectobacterium brasiliense]
ATGAGCTATAGCGTCAAATTCAGGGAAGATGCGCTGAAAGAATGGCTCAAGCTCGATAAAACCATCCAGCAGCAATTTGC
CAAAAAACTAAAAAAATGCTGCGAAAATCCCCATATTCCTTCAGCAAAGTTACGGGGGATGAAGGACTGCTACAAAATAA
AGCTCCGAGCGTCAGGCTTTCGTTTGGTTTATGAGGTTATTGACGACGTATTGATTATCGCCGTAGTGGCGGTAGGTAAA
CGTGAGCGCAGCGGTGTTTATCATCTGGCGAGTGAGCGAATGAGATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT141420 WP_000581937.1 NZ_CP045206:1047988-1048236 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT141420 NZ_CP059959:1221215-1221466 [Pectobacterium brasiliense]
ATGCCGAATATTATATTGAGTGATACCAGTGCCAGCGTCAGTGAACTGAAAAAGAACCCAATGGCGACGGTCAATGCTGG
CGATGGCCTGCCTGTTGCTATCTTGAACCGCAACCAGCCTGCGTTTTATTGCATTCCTGCGGATCTCTACGAAAAAATGC
TGGATGCGCTGGACGATCAAGAACTGGTTAAGCTAATCGGTGAACGCAAAAACCAGCCGCTGGTTGACGTGGATTTGGAT
AGTTTCCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References